Simultaneous determination of the novel neuroprotective agent KR-31378 and its metabolite KR-31612 using high performance liquid chromatography with tandem mass spectrometry in human plasma.

Abstract

An LC/MS/MS method for the simultaneous determination of a neuroprotective agent for ischemia-reperfusion damage, KR-31378 and its N-acetyl metabolite KR-31612 in human plasma was developed. KR-31378, KR-31612 and the internal standard, KR-31543 were extracted from human plasma by liquid-liquid extraction. A reverse-phase HPLC separation was performed on Luna phenylhexyl column with the mixture of acetonitrile-5 mM ammonium formate (55:45, v/v) as mobile phase. The detection of analytes was performed using an electrospray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The lower limits of quantification for KR-31378 and KR-31612 were 2.0 ng/ml. The method showed a satisfactory sensitivity, precision, accuracy, recovery and selectivity.

• Authors: John Ki, Hye Young Ji, Sung-Eun Yoo, Sun Ok Kim, Dong Ha Lee, Hong Lim, Hye Suk Lee
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 25 Issue 5 Pg. 647-51 (Oct 2002) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 12433199 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzopyrans
• Guanidines
• KR-31612
• N''-cyano-N-(6-amino-3,4-dihydro-3-hydroxy-2-methyl-2-dimethoxymethyl-2H-benzopyran-4-yl)-N'-benzylguanidine
• Neuroprotective Agents
• Pyrans

Topics

• Benzopyrans (blood, chemistry)
• Chromatography, High Pressure Liquid (methods)
• Gas Chromatography-Mass Spectrometry (methods)
• Guanidines (blood, chemistry, pharmacology)
• Humans
• Neuroprotective Agents (blood, chemistry)
• Pyrans (blood, chemistry, pharmacology)