Chemokines modulate experimental autoimmune thyroiditis through attraction of autoreactive or regulatory T cells.

Abstract

A critical event in the pathogenesis of experimental autoimmune thyroiditis (EAT) is the entry of thyroid-specific T lymphocytes into the thyroid gland. To investigate the role of soluble mediators in that infiltration, we have assayed the expression of various chemokines in diseased thyroid glands and in cytokine-treated cultures of normal thyroid epithelial cells. MCP-1 (monocyte chemotactic protein-1) and RANTES are produced during EAT and induced in vitro by IFN-gamma, IL-10, TNF-alpha, and IL-1beta. In vitro chemotaxis experiments using immune lymph node (LN) cells showed that RANTES attracted mTg-specific responder LN cells, whereas MCP-1 attracted mTg-specific CD4(+), CD25(+) regulator cells that secreted IL-10. The in vivo transfer of LN T cells attracted in vitro either by RANTES or by MCP-1 confirmed their opposite effects on the course of EAT.

• Authors: Claire Goulvestre, Frédéric Batteux, Jeannine Charreire
• Journal: European journal of immunology (Eur J Immunol) Vol. 32 Issue 12 Pg. 3435-42 (Dec 2002) ISSN: 0014-2980 [Print] Germany
• PMID: 12432574 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL2
• Chemokine CCL5
• Chemokines
• Interleukin-1
• Receptors, Interleukin-2
• Recombinant Proteins
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Interferon-gamma

Topics

• Adoptive Transfer
• Animals
• Autoimmunity
• CD4-Positive T-Lymphocytes (immunology)
• Cells, Cultured
• Chemokine CCL2 (biosynthesis)
• Chemokine CCL5 (biosynthesis)
• Chemokines (biosynthesis, genetics)
• Chemotaxis
• Epithelial Cells (drug effects, immunology)
• Female
• In Vitro Techniques
• Interferon-gamma (pharmacology)
• Interleukin-1 (pharmacology)
• Interleukin-10 (biosynthesis, pharmacology)
• Mice
• Mice, Inbred C57BL
• Mice, Inbred CBA
• Receptors, Interleukin-2 (metabolism)
• Recombinant Proteins
• T-Lymphocyte Subsets (immunology)
• T-Lymphocytes (immunology)
• Thyroid Gland (drug effects, immunology)
• Thyroiditis, Autoimmune (etiology, genetics, immunology)
• Tumor Necrosis Factor-alpha (pharmacology)