[Alkaline phosphatase isoenzymes in the serum of patients with renal insufficiency].

Abstract

OBJECTIVE:

The aim of this study was to test the utility of serum alkaline phosphatase isoenzymes determination from patients with renal insufficiency.

MATERIAL AND METHODS:

Serum levels of alkaline phosphatase isoenzymes were determined in a group of 58 patients: 22 of them suffering acute renal insufficiency (ARI) and 36 with chronic renal failure (CRF) undergoing regular hemodialysis, results obtained were compared from a population of 30 healthy adults. Intestinal, bone, liver, macromolecular and intestinal variant isoenzymes, were separated by electrophoresis on agarose gel and quantified using a densitometer.

RESULTS:

Were found a significant increase the total alkaline phosphatase activity in both pathologic groups (p < 0.05 in ARI and p < 0.01 in CRF). Isoenzymatic profiles showed: increase of the bone fraction (p < 0.05 in ARI and p < 0.001 in CRF), decrease of the liver isoenzyme (p < 0.001) in both groups, macromolecular fraction elevated in acute patients (p < 0.05) and a significant increase of the intestinal and intestinal variant isoenzyme in the chronic patients (p < 0.001).

CONCLUSION:

The renal insufficiency modified the normal distribution of alkaline phosphatase isoenzymes and the study of their serum levels could be an effective non-invasive marker, for the evaluation of bone disease and intestinal disorders associated with renal failure.

• Authors: M R Sánchez Navarro, M E Fernández-Conde, S Blanco Martín, C Samaniego
• Journal: Anales de medicina interna (Madrid, Spain : 1984) (An Med Interna) Vol. 19 Issue 9 Pg. 449-52 (Sep 2002) ISSN: 0212-7199 [Print] Spain
• Vernacular Title: Isoenzimas de fosfatasa alcalina en el suero de pacientes con insuficiencia renal.
• PMID: 12420628 (Publication Type: English Abstract, Journal Article)

Chemical References

• Isoenzymes
• Alkaline Phosphatase

Topics

• Adult
• Aged
• Alkaline Phosphatase (blood)
• Bone Diseases (blood, etiology)
• Female
• Gastrointestinal Diseases (blood, etiology)
• Humans
• Isoenzymes (blood)
• Male
• Middle Aged
• Renal Dialysis
• Renal Insufficiency (blood, complications, therapy)