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Presence of avipoxvirus DNA in avian dermal squamous cell carcinoma.

Abstract
Dermal squamous cell carcinoma (DSCC; avian keratoacanthoma) is a neoplastic skin lesion of broiler chickens of unknown aetiology. In previous studies, the possibility of the involvement of pox viruses in the cause of DSCC was considered. In this work, a sensitive and specific nested polymerase chain reaction (PCR) protocol was developed that could amplify a 419 base pair DNA fragment of fowlpox virus with a detection limit of less than one infectious unit. Fowlpox virus DNA was always detected in skin samples with fowlpox lesions while it was not detected in samples of unrelated diseases such as cowpox, Marek's disease or infectious laryngotracheitis. Some macroscopically normal skin samples from vaccinated and non-vaccinated birds also produced PCR-positive results, corroborating previous studies on the possibility that a latent or chronic form of fowlpox occurs. Fowlpox virus DNA was consistently detected from DSCC skin lesions, and this finding is discussed.
AuthorsLuiz C B Fallavena, Cláudio W Canal, Carlos T P Salle, Hamilton L S Moraes, Silvio L S Rocha, Rosecler A Pereira, Ari B da Silva
JournalAvian pathology : journal of the W.V.P.A (Avian Pathol) Vol. 31 Issue 3 Pg. 241-6 (Jun 2002) ISSN: 0307-9457 [Print] England
PMID12396347 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA Primers
  • DNA, Viral
Topics
  • Animals
  • Avipoxvirus (genetics, isolation & purification)
  • Carcinoma, Squamous Cell (pathology, veterinary, virology)
  • Chick Embryo
  • Chickens
  • DNA Primers
  • DNA, Viral (isolation & purification)
  • Nucleic Acid Amplification Techniques (veterinary)
  • Polymerase Chain Reaction (methods, veterinary)
  • Poultry Diseases (pathology, virology)
  • Sensitivity and Specificity
  • Skin Neoplasms (pathology, veterinary, virology)
  • Specific Pathogen-Free Organisms

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