Abstract |
To study an involvement of glomerular endothelial cells in the development of anti-Thy-1 nephritis, we examined the expression of endothelial cell adhesion molecules during the course of this model. Ribonuclease protection assay elucidated that expression of mRNA for intercellular adhesion molecule-1 (ICAM-1) was markedly enhanced in the glomeruli with a peak at 2 h (6.5-fold, p < 0.05) after the anti-Thy-1 antibody injection when mesangial cell lysis was recognized and IL-1beta mRNA expression was induced in the glomeruli. The glomerular ICAM-1 was predominantly localized in the endothelial cells and was intensely immunostained at day 1 in the glomerular endothelial cells. In contrast, platelet endothelial cell adhesion molecule-1 (PECAM-1) and vascular endothelial-cadherin mRNA expression increased gradually with a peak at day 6 (2.6-fold (p < 0.05) and 4.2-fold (p < 0.05), respectively) in the glomeruli with mesangial proliferative lesion. PECAM-1 was also immunolocalized in the glomerular endothelial cells and the immunoreactivity was greatly enhanced at day 6. Glomerular expression of vascular cell adhesion molecule-1 and endothelial leukocyte adhesion molecule-1 ( E-selectin) was unchanged at a low level during the course of anti-Thy-1 nephritis. Blocking of ICAM-1 by administration of anti-ICAM-1 antibody showed significant decrease in the number of polymorphonuclear leukocytes accumulating in the glomeruli by 45.7% (9.4 +/- 0.2 vs. 5.1 +/- 0.1 per glomerular cross section, p < 0.01) at 2 h. These results suggest a significant involvement of glomerular endothelial cells in the development and repair of anti-Thy-1 nephritis via direct or indirect intercellular interactions between mesangial cells and glomerular endothelial cells.
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Authors | Masato Isome, Hidehiko Fujinaka, Eishin Yaoita, Lili Feng, Laxman P Adhikary, Akira Abe, Satoko Tsuchida, Katsutoshi Kawasaki, Hitoshi Suzuki, Itaru Kihara, Curtis B Wilson, Tadashi Yamamoto |
Journal | Experimental nephrology
(Exp Nephrol)
Vol. 10
Issue 5-6
Pg. 338-47
( 2002)
ISSN: 1018-7782 [Print] Switzerland |
PMID | 12381918
(Publication Type: Journal Article)
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Copyright | Copyright 2002 S. Karger AG, Basel |
Chemical References |
- Antibodies
- Cell Adhesion Molecules
- Inflammation Mediators
- Isoantibodies
- RNA, Messenger
- anti-Thy antibody
- Intercellular Adhesion Molecule-1
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Topics |
- Animals
- Antibodies
(pharmacology)
- Cell Adhesion Molecules
(genetics, metabolism)
- Endothelium
(metabolism)
- Female
- Immunologic Techniques
- Inflammation Mediators
(metabolism)
- Intercellular Adhesion Molecule-1
(immunology)
- Isoantibodies
(immunology)
- Kidney Glomerulus
(metabolism)
- Nephritis
(immunology, urine)
- Neutrophil Infiltration
(drug effects, immunology)
- Proteinuria
(etiology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Inbred WKY
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