Abstract | BACKGROUND/AIM: METHODS: Isolated perfused rat livers were subjected to 1 h of warm ischemia followed by 90 min of reperfusion without (n = 5) or with GSH or catalase (n = 4-5 each). Selective KC activation by zymosan (150 micro g/ml) in continuously perfused rat livers was used to investigate KC-related liver injury. RESULTS: Postischemic infusion of 0.1, 0.5, 1.0 and 2.0 mM GSH, but not 0.05 mM GSH prevented reperfusion injury after warm ischemia as indicated by a marked reduction of sinusoidal LDH efflux by up to 83 +/- 13% (mean +/- SD; p < 0.05) and a concomitant significant improvement of postischemic bile flow by 58 +/- 27% (p < 0.05). A similar protection was conveyed by KC blockade with gadolinium chloride indicating prevention of KC-related reperfusion injury by postischemic GSH treatment. Postischemic treatment with catalase (150 U/ml) resulted in a reduction of LDH efflux by 40 +/- 9% (p < 0.05). Accordingly, catalase as well as GSH (0.1-2.0 mM) nearly completely prevented the increase in LDH efflux following selective KC activation by zymosan in continously perfused rat livers. CONCLUSION:
|
Authors | Manfred Bilzer, Andreas Baron, Rolf Schauer, Christian Steib, Stefan Ebensberger, Alexander L Gerbes |
Journal | Digestion
(Digestion)
Vol. 66
Issue 1
Pg. 49-57
( 2002)
ISSN: 0012-2823 [Print] Switzerland |
PMID | 12379815
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2002 S. Karger AG, Basel |
Chemical References |
- Antioxidants
- Zymosan
- Glutathione
|
Topics |
- Animals
- Antioxidants
(pharmacology)
- Glutathione
(pharmacology)
- Kupffer Cells
(metabolism)
- Liver
(blood supply)
- Male
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
- Zymosan
(pharmacology)
|