Effect of synthetic oligopeptides on osteoporosis.

Abstract

The objective of this study was to establish the solution method of GHRPS, the synthetic oligopeptides Tyr-Gly-Gly-Phe-Met-NH2, Tyr-Gly-Gly-Phe-Met-OH, Tyr-Gly-Gly-Phe-Leu-NH2, Tyr-Gly-Gly-Phe-Leu-OH, Tyr-Gly-Gly-Phe-Gly-NH2, and Tyr-Gly-Gly-Phe-Gly-OH, to verify their effect on osteoporosis. Male ICR mice (20+/-2 g) were used. The intramuscular injection dose of 6.3 mg/kg prednisone induced a significant decrease of body and femur weight of the animals. The subcutaneous injection dose of 18 microg/kg synthetic peptide was not effective to prevent the decrease of body and femur weight of the animals. The subcutaneous injection dose of 6.3 mg/kg prednisone elicited a decrease in content of femur calcium and in the level of serum calcium of the animals. The subcutaneous injection dose of 18 microg/kg Tyr-Gly-Gly-Phe-Leu-NH2, or Tyr-Gly-Gly-Phe-Leu-OH, or Tyr-Gly-Gly-Phe-Gly-NH2 significantly increased the content of femur calcium and decreased the level of serum calcium of the animals. It was also observed that the subcutaneous injection dose of 18 microg/kg Tyr-Gly-Gly-Phe-Gly-OH, Tyr-Gly-Gly-Phe-Leu-OH, Tyr-Gly-Gly-Phe-Met-OH, Tyr-Gly-Gly-Phe-Met-NH2 significantly increased the content of femur phosphorous and decreased the activity of ALP of the animals.

• Authors: Weina Cui, Chao Wang, Ming Zhao, Shiqi Peng
• Journal: Preparative biochemistry & biotechnology (Prep Biochem Biotechnol) Vol. 32 Issue 3 Pg. 253-68 (Aug 2002) ISSN: 1082-6068 [Print] England
• PMID: 12375813 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligopeptides
• Growth Hormone-Releasing Hormone
• Calcium

Topics

• Animals
• Bone and Bones (drug effects)
• Calcium (blood)
• Growth Hormone-Releasing Hormone (analogs & derivatives, genetics, pharmacology)
• Male
• Mice
• Mice, Inbred ICR
• Oligopeptides (chemical synthesis, chemistry, pharmacology)
• Osteoporosis (drug therapy)