Ontogenetic development and spatial distribution of the ileal apical sodium-dependent bile acid transporter and the ileal lipid-binding protein in apoE knockout and C57BL/6 mice.

Abstract

BACKGROUND:

Although apoE-/- mice are characterized by hypercholesterolemia, the bile acid enterohepatic circulation, which plays a crucial role in cholesterol homeostasis, has not been examined in these mice. The differences between apoE-/- and C57BL/6 mice in expression of the ileal ASBT and ILBP and in intestinal bile acid absorption were studied.

METHODS:

The intestinal tissues of the fetal, neonatal and post-weaning mice were processed for immunohistochemistry. Body retention and fecal excretion of 75SeHCAT were measured. The bile acid pool size and its composition were analysed by HPLC.

RESULTS:

In apoE-/- and C57BL/6 mice, the bile acid pool size was 75 +/- 13 and 78 +/- 13 micromol/ 100 g body weight, respectively, while the ratio of cholic acid/beta-muricholic acid was 1.8 +/- 0.3 and 1.4 +/- 0.3 (P < 0.05), respectively. The daily body retention of 75SeHCAT was 48% = 1.8% in C57 black mice and 58.4% +/- 2.7% in apoE-/- mice (P < 0.05). In both mouse strains, ASBT expression in the small intestine was found in the near-term fetal and post-weaning mice, while ILBP expression was found in all postnatal mice. In the post-weaning mice, ILBP expression was limited to the distal 25%-30% of the small intestine, while ASBT expression was limited to the distal 18%.

CONCLUSIONS:

The bile acid enterohepatic circulation in apoE-/- mice probably does not differ greatly from that in C57BL/6 mice.

• Authors: P Håkansson, I Andersson, S Nyström, L Löfgren, L F Amrot, Hong Li
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 37 Issue 9 Pg. 1089-96 (Sep 2002) ISSN: 0036-5521 [Print] England
• PMID: 12374235 (Publication Type: Journal Article)

Chemical References

• Apolipoproteins E
• Bile Acids and Salts
• Carrier Proteins
• Cholic Acids
• Organic Anion Transporters, Sodium-Dependent
• Symporters
• sodium-bile acid cotransporter
• muricholic acid
• Cholesterol
• Cholic Acid

Topics

• Animals
• Apolipoproteins E (physiology)
• Bile Acids and Salts (metabolism)
• Carrier Proteins (metabolism)
• Cholesterol (blood)
• Cholic Acid (metabolism)
• Cholic Acids (metabolism)
• Female
• Ileum (embryology, growth & development, metabolism)
• Immunoenzyme Techniques
• Intestinal Absorption (physiology)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Organic Anion Transporters, Sodium-Dependent
• Symporters