Abstract | AIMS/HYPOTHESIS: METHODS: The radiolabeled proteins were delivered to the circulation of conscious diabetic (STZ induced for 6 weeks) and control rats by ALZET osmotic pumps. The plasma level of the radiolabeled proteins reached steady-state levels by day 7. Urine and plasma samples from day 7 were used to determine the excretion rates of the proteins by radioactivity and radioimmunoassay. RESULTS: When excretion rates were determined by radioactivity it was apparent that only the albumin excretion rate increased significantly with STZ diabetes to a value of 354 +/- 166 microg/min which agrees with proteinuria determined by Biuret assay of 299.9 +/- 52.4 microg/min. The major proportion of protein being excreted was in the form of protein fragments which are not detected by conventional immmunochemical assays. CONCLUSION: The previously unrecognized nephrotic-like levels of proteinuria in experimental diabetes appears to be associated with an albumin-specific mechanism responsible for the increase in albumin peptides in urine. There was significant lowering of plasma albumin concentration but plasma concentrations of transferrin and immunoglobulin G remained unchanged. There was also no significant appearance of intact protein in urine that is normally found in nephrotic states.
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Authors | Kerryn A Greive, Tanya M Osicka, Leileata M Russo, Wayne D Comper |
Journal | American journal of nephrology
(Am J Nephrol)
2003 Jan-Feb
Vol. 23
Issue 1
Pg. 38-46
ISSN: 0250-8095 [Print] Switzerland |
PMID | 12373079
(Publication Type: Journal Article)
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Copyright | Copyright 2003 S. Karger AG, Basel |
Topics |
- Animals
- Diabetes Mellitus, Experimental
(physiopathology)
- Male
- Nephrotic Syndrome
(physiopathology)
- Proteinuria
(physiopathology)
- Radioimmunoassay
- Rats
- Rats, Sprague-Dawley
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