Detection of the genes induced in activated lymphocytes by modified differential display.

Abstract	Activated lymphocytes induced by mitogens or antigens express various sets of genes, including those involved in the expression of cytokines, surface molecules, and nuclear proteins. To detect inducible genes in activated lymphocytes, we used the RNA arbitrarily primed polymerase chain reaction (RAP-PCR) method, which is modified by original differential display. By this method we identified eight clones; four contained sequences almost identical to that of the genes for heat shock 90Kd protein1 alpha, STAT2, Ig kappa constant region and interferon receptor 1, two had 70% homology to mitochondrial ATP synthase and bromodomain-containing 2 genes, and two had less than 40% homology to known DNA sequences. By RT-PCR, the heat shock 90 Kd protein1 alpha, STAT2 and interferon receptor 1 genes showed increased expression in phytohemagglutinin (PHA)-stimulated or antigen stimulated T cell line. Differential display is a useful method for detection of inducible genes from a small amount of material and at various time points, although the homology of PCR primer influences the displayed genes.
Authors	Hideo Kaneko, Ryosuke Inoue, Takahide Teramoto, Wataru Morimoto, Kouji Isogai, Kimiko Kasahara, Naomi Kondo
Journal	Journal of investigational allergology & clinical immunology (J Investig Allergol Clin Immunol) Vol. 12 Issue 2 Pg. 86-90 ( 2002) ISSN: 1018-9068 [Print] Spain
PMID	12371535 (Publication Type: Journal Article)
Chemical References	BRD3 protein, human DNA Primers DNA-Binding Proteins HSP90 Heat-Shock Proteins Immunoglobulin Constant Regions Phytohemagglutinins RNA-Binding Proteins Receptors, Interferon STAT2 Transcription Factor STAT2 protein, human Trans-Activators Transcription Factors
Topics	Base Sequence (genetics) Cell Differentiation (genetics) Cell Division (genetics) DNA Primers (genetics) DNA-Binding Proteins (genetics) Gene Expression Profiling HSP90 Heat-Shock Proteins (genetics) Humans Immunoglobulin Constant Regions (genetics) Leukocytes, Mononuclear (physiology) Lymphocyte Activation (genetics) Lymphocytes (cytology) Phytohemagglutinins (pharmacology) RNA-Binding Proteins (genetics) Receptors, Interferon (genetics) STAT2 Transcription Factor Trans-Activators (genetics) Transcription Factors Up-Regulation (genetics)

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