Abstract |
There is strong evidence in many tumor types, including thyroid cancer, for a novel tumor suppressor gene (TSG) at 17p13. To identify the putative thyroid 17p13 TSG we mapped thyroid tumor loss of heterozygosity (LOH) at high resolution within this region. We examined 20 typical follicular thyroid carcinomas ( FTC), 19 Hurthle cell carcinomas (HCC), 15 papillary thyroid carcinomas (PTC), and 7 follicular adenomas (FA) for LOH at 17p13 using 18 probes. Complete clinical follow-up data were available for all patients. We confirmed a high 17p13 LOH rate in FTC (18 of 20) and HCC (13 of 19) and showed an association between 17p13 LOH and advanced tumor grade. Only 4 of 15 PTC and 1 of 7 FA displayed 17p13 LOH. In the HCC we identified a narrow minimal common deleted region between D17S1308 (285 kb from the p-telomer) and D17S695 (696 kb from the p-telomer). This region was flanked centromerically by a breakpoint cluster, further suggesting nonrandom deletion. All but 1 of the PTC and FA with 17p LOH and 50% of the affected FTC also showed LOH in this region. These data suggest that a TSG, involved in HCC pathogenesis, is contained within the D17S1308-D17S695 interval. There are several potential candidate TSGs in this region that are worthy of further study.
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Authors | Kathryn Farrand, Brett Delahunt, Xiao-Li Wang, Bryan McIver, Ian D Hay, John R Goellner, Norman L Eberhardt, Stefan K G Grebe |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 87
Issue 10
Pg. 4715-21
(Oct 2002)
ISSN: 0021-972X [Print] United States |
PMID | 12364463
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Adenocarcinoma, Follicular
(genetics, pathology)
- Adenoma, Oxyphilic
(genetics, pathology)
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Carcinoma, Papillary
(genetics, pathology)
- Chromosome Mapping
- Chromosomes, Human, Pair 17
- Female
- Gene Deletion
- Genes, Tumor Suppressor
- Humans
- Loss of Heterozygosity
- Male
- Microsatellite Repeats
- Middle Aged
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Thyroid Neoplasms
(genetics, pathology)
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