Receptors for recombinant feline interferon-omega in hemocytes of the Japanese pearl oyster Pinctada fucata martensii.

Abstract

In a previous study we determined that administration of recombinant feline interferon-omega (rFeIFN-omega) could protect Japanese pearl oysters Pinctada fucata martensii from akoya-virus infection. Our results suggested that rFeIFN-omega enhanced the potential of agranulocytes to phagocytize necrotic cells and to produce collagen fibers that repair the lesions caused by the akoya-virus. In the present study, using an indirect immunofluorescence technique with an anti-rFeIFN-omega rabbit serum, we examined whether hemocytes bear receptors that bind rFeIFN-omega. rFeIFN-omega receptors were present on agranulocytes and bound rFeIFN-omega, and appeared as green fluorescent spots in the cytoplasm under a fluorescent microscope. Around 56% of agranulocytes in Japanese pearl oysters bore the rFeIFN-omega receptors.

• Authors: Teruo Miyazaki, Tomohito Taniguchi, Jinto Hirayama, Naoki Nozawa
• Journal: Diseases of aquatic organisms (Dis Aquat Organ) Vol. 51 Issue 2 Pg. 135-8 (Aug 29 2002) ISSN: 0177-5103 [Print] Germany
• PMID: 12363085 (Publication Type: Journal Article)

Chemical References

• Interferon Type I
• Receptors, Interferon
• interferon omega 1

Topics

• Animals
• Fluorescent Antibody Technique, Indirect (veterinary)
• Hemocytes (immunology, metabolism, ultrastructure)
• Histocytochemistry (veterinary)
• Interferon Type I (genetics, immunology, metabolism)
• Japan
• Microscopy, Fluorescence (veterinary)
• Ostreidae (immunology, metabolism)
• Receptors, Interferon (metabolism)