Quantitative analysis of the novel depsipeptide anticancer drug Kahalalide F in human plasma by high-performance liquid chromatography under basic conditions coupled to electrospray ionization tandem mass spectrometry.

Kahalalide F (KF) is a novel cyclic depsipeptide anticancer drug, which has shown anticancer activity both in vitro and in vivo especially against human prostate cancer cell lines. To characterize the pharmacokinetics of KF during a phase I clinical trial in patients with androgen refractory prostate cancer, a method was developed and validated for the quantitative analysis of KF in human plasma using high-performance liquid chromatography (HPLC) coupled to positive electrospray ionization tandem mass spectrometry (ESI-MS/MS). Microbore reversed-phase liquid chromatography (LC) performed with mobile phases containing trifluoroacetic acid, an additive commonly used for separating peptides, resulted in substantial suppression of the signal for KF on ESI-MS/MS. An alternative approach employing a basic mobile phase provided an excellent response for KF when detected in the positive ion mode. Plasma samples were prepared for LC MS/MS by solid-phase extraction on C(18) cartridges. The LC separation was performed on a Zorbax Extend C(18) column (150 x 2.1 mm i.d., particle size 5 micro m) with acetonitrile -10 mM aqueous ammonia (85 : 15, v/v) as the mobile phase, at a flow-rate of 0.20 ml min(-1). A butyric acid analogue of KF was used as the internal standard. The lower limit of quantitation (LLQ) using a 500 micro l sample volume was 1 ng ml(-1) and the linear dynamic range extended to 1000 ng ml(-1). The inter-assay accuracy of the assay was -15.1% at the LLQ and between -2.68 and -9.05% for quality control solutions ranging in concentration from 2.24 to 715 ng ml(-1). The inter-assay precision was 9.91% or better at these concentrations. The analyte was stable in plasma under all relevant conditions evaluated and for a period of 16 h after reconstituting plasma extracts for LC analysis at ambient temperature.
AuthorsE Stokvis, H Rosing, L López-Lázaro, I Rodriguez, J M Jimeno, J G Supko, J H M Schellens, J H Beijnen
JournalJournal of mass spectrometry : JMS (J Mass Spectrom) Vol. 37 Issue 9 Pg. 992-1000 (Sep 2002) ISSN: 1076-5174 [Print] England
PMID12271442 (Publication Type: Clinical Trial, Clinical Trial, Phase I, Journal Article)
CopyrightCopyright 2002 John Wiley & Sons, Ltd.
Chemical References
  • Antineoplastic Agents
  • Depsipeptides
  • Peptides
  • kahalalide F
  • Antineoplastic Agents (blood, pharmacokinetics)
  • Area Under Curve
  • Calibration
  • Chromatography, High Pressure Liquid
  • Depsipeptides
  • Drug Stability
  • Humans
  • Peptides (blood, pharmacokinetics)
  • Quality Control
  • Reference Standards
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization

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