Abstract | BACKGROUND: METHODS: Thirty-four fresh specimens of transrectal prostatic needle biopsy were obtained; 11 were pathologically diagnosed as adenocarcinoma and 23 as without malignancy. The gene expression levels of five isozymes (type 1-5) of 17betaHSD were evaluated. The quantification of gene expression was assessed by means of the real-time polymerase chain reaction. RESULTS: The expression levels of the type 3 17betaHSD gene with malignancy were significantly higher than those in prostatic tissues without malignancy, and those of type 2 17betaHSD with malignancy were significantly lower than those in nonmalignant tissues. There were no significant differences in 17betaHSD type 1, type 4, and type 5 gene expression in cancerous and noncancerous tissues. CONCLUSION: Our results suggest that 17betaHSD type 2 and type 3 play an important role in the conversion of adrenal steroids into potential androgens in prostate cancer tissue.
|
Authors | Eitetsu Koh, Toru Noda, Jiro Kanaya, Mikio Namiki |
Journal | The Prostate
(Prostate)
Vol. 53
Issue 2
Pg. 154-9
(Oct 01 2002)
ISSN: 0270-4137 [Print] United States |
PMID | 12242730
(Publication Type: Comparative Study, Journal Article)
|
Copyright | Copyright 2002 Wiley-Liss, Inc. |
Chemical References |
- Isoenzymes
- RNA, Neoplasm
- 17-Hydroxysteroid Dehydrogenases
- 3 (or 17)-beta-hydroxysteroid dehydrogenase
|
Topics |
- 17-Hydroxysteroid Dehydrogenases
(biosynthesis, genetics)
- Adenocarcinoma
(enzymology, genetics)
- Biopsy, Needle
- Gene Expression
- Humans
- Isoenzymes
(biosynthesis, genetics)
- Male
- Prostatic Neoplasms
(enzymology, genetics)
- RNA, Neoplasm
(chemistry, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Statistics, Nonparametric
|