Abstract |
Radiation therapy is an established modality for the treatment of malignant gliomas. Several reports have shown the advantage of additional radiation in combination with gene therapy. In this study, we investigated the ability of radiation therapy to enhance 5-fluorocytosine (5-FC)/ cytosine deaminase (CD) plus uracil phosphoribosyltransferase (UPRT) gene therapy in malignant gliomas. In vitro study suggested evidence of a significant cytotoxic interaction between radiation therapy and 5-FC/CD + UPRT gene therapy for glioma cells. In vivo experiments demonstrated that the combination of gene therapy and radiation possessed superior antitumor effect in comparison to single therapy. However, the adverse effects of radiation therapy in combination with the gene therapy were observed with respect to normal brain. This combination therapy may be feasible for the treatment of gliomas, although the radiation dose and area should be reduced in order to prevent side effects.
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Authors | Hirokazu Kambara, Takashi Tamiya, Yasuhiro Ono, Shinji Ohtsuka, Kinya Terada, Yoshiaki Adachi, Tomotsugu Ichikawa, Hirofumi Hamada, Takashi Ohmoto |
Journal | Cancer gene therapy
(Cancer Gene Ther)
Vol. 9
Issue 10
Pg. 840-5
(Oct 2002)
ISSN: 0929-1903 [Print] England |
PMID | 12224025
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Tetrazolium Salts
- Thiazoles
- Pentosyltransferases
- uracil phosphoribosyltransferase
- Nucleoside Deaminases
- Cytosine Deaminase
- thiazolyl blue
- Fluorouracil
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Topics |
- Adenoviridae
(genetics)
- Animals
- Apoptosis
- Brain
(radiation effects)
- Brain Neoplasms
(enzymology, pathology, therapy)
- Cell Division
- Combined Modality Therapy
- Cytosine Deaminase
- Flow Cytometry
- Fluorouracil
(therapeutic use)
- Gene Transfer Techniques
- Genetic Therapy
(methods)
- Genetic Vectors
- Gliosarcoma
(enzymology, pathology, therapy)
- Lac Operon
- Male
- Nucleoside Deaminases
(genetics, metabolism)
- Pentosyltransferases
(genetics, metabolism)
- Radiotherapy
(methods)
- Rats
- Rats, Wistar
- Survival Rate
- Tetrazolium Salts
- Thiazoles
- Tumor Cells, Cultured
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