Primary sensory neurons: a common final pathway for inflammation in experimental pancreatitis in rats.

We hypothesized that neurogenic inflammation is a common final pathway for parenchymal inflammation in pancreatitis and evaluated the role of primary sensory neurons in secretagogue-induced and obstructive pancreatitis. Neonatal rats received either the primary sensory neuron-denervating agent capsaicin (50 mg/kg s.c.) or vehicle. At 8 wk of age, pancreatitis was produced by six hourly injections of caerulein (50 microg/kg i.p.) or by common pancreaticobiliary duct ligation (CPBDL). The severity of pancreatitis was assessed by serum amylase, pancreatic myeloperoxidase (MPO) activity, histological grading, pancreatic plasma extravasation, and wet-to-dry weight ratio. Caerulein significantly increased MPO activity and wet-to-dry weight ratio, produced histological evidence of edematous pancreatitis, induced plasma extravasation, and caused hyperamylasemia. CPBDL increased MPO activity and produced histological evidence of pancreatitis. Neonatal capsaicin administration significantly reduced tissue MPO levels, histological severity scores, and wet-to-dry weight ratio and abolished plasma extravasation. These results demonstrate that primary sensory neurons play a significant role in the inflammatory cascade in experimental pancreatitis and appear to constitute a common final pathway for pancreatic parenchymal inflammation.
AuthorsJaimie D Nathan, Ruth Y Peng, Yu Wang, Douglas C McVey, Steven R Vigna, Rodger A Liddle
JournalAmerican journal of physiology. Gastrointestinal and liver physiology (Am J Physiol Gastrointest Liver Physiol) Vol. 283 Issue 4 Pg. G938-46 (Oct 2002) ISSN: 0193-1857 [Print] United States
PMID12223354 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Ceruletide
  • Peroxidase
  • Amylases
  • Capsaicin
  • Amylases (blood)
  • Animals
  • Animals, Newborn
  • Bile Ducts (surgery)
  • Capsaicin (administration & dosage)
  • Ceruletide (administration & dosage)
  • Denervation
  • Ligation
  • Male
  • Neurons, Afferent (physiology)
  • Pancreas (enzymology, innervation, pathology)
  • Pancreatic Ducts (surgery)
  • Pancreatitis (etiology, pathology, physiopathology)
  • Peroxidase (blood)
  • Rats
  • Rats, Sprague-Dawley

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: