We developed a new technique for culture study that successfully recovers fungi from
drug-treated skin tissues, in which tissue specimens were homogenized, dialyzed against water, digested with
trypsin, and then washed with PBS, to eliminate the
drug that remaining in the skin tissue specimens. With this modified culture method, we reevaluated the efficacy of
KP-103,
neticonazole, and
lanoconazole in a guinea pig interdigital
tinea pedis model. Guinea pigs with
tinea pedis were topically treated with a 1%
solution of
KP-103 or a reference
drug once a day for 10 consecutive days. Five days after the last treatment, left and right feet were subjected to culture study by the conventional and modified recovery culture methods, respectively. One hundred percent (20/20) of
lanoconazole-treated feet were judged as culture-negative by the conventional culture method, but 85% (17/20) of the feet were shown to be culture-positive when the modified recovery culture method was used. On the other hand,
KP-103 achieved high rates of culture-negative rates, 95% (19/20) and 85% (17/20), in both conventional and modified culture methods, respectively. Furthermore, on day-30 posttreatment,
KP-103 sterilized 14 of the 20 infected feet, whereas
neticonazole and
lanoconazole were not effective even in reducing fungal burden.
KP-103 proved to be highly effective in achieving mycological cure and preventing relapse against
tinea pedis presumably because of its good bioavailability in the skin based on its low
keratin-affinity, along with its potent antifungal activity.