Inhibitory effect of BCG cell-wall skeletons (BCG-CWS) emulsified in squalane on tumor growth and metastasis in mice.

Abstract

The antimetastatic effect of BCG-CWS, which was emulsified in an oil-in-water form with either Drakeol 6VR mineral oil (BCG-CWS/DK) or squalane (BCG-CWS/SQA), on lung metastasis produced by highly metastatic murine tumor cells, Colon26-M3.1 carcinoma cells and B16-BL6 melanoma cells, was investigated in syngeneic mice. An intravenous (i.v.) administration of BCG-CWS (100 mg/mouse) 1 day after tumor inoculation significantly inhibited tumor metastasis of both Colon26-M3.1 carcinoma and B16-BL6 melanoma cells in experimental lung metastasis models. No differences in the antitumor activity of the two oil-based formulations (BCG-CWS/DK and BCG-CWS/SQA) were obverved. However, BCG-CWS/SQA administered through subcutaneous (s.c.) route was shown to be effective only when it was consecutively injected (3 times) after tumor inoculation. An in vivo analysis for tumor-induced angiogenesis showed that a single i.v. administration of BCG-CWS/SQA inhibited the number of tumor-induced blood vessels and suppressed tumor growth. Furthermore, the multiple administration of BCG-CWS/SQA given at on week intervals led to a significant reduction in spontaneous lung metastasis of B16-BL6 melanoma cells in a spontaneous metastasis model. These results suggest that BCG-CWS emulsified with squalane is a potent inhibitory agent of lung metastasis, and that the antimetastatic effect of BCG-CWS is related to the suppression of tumor growth and the inhibition of tumor-induced angiogenesis.

• Authors: Yung Choon Yoo, Katsusuke Hata, Kyung Bok Lee, Ichiro Azuma
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 25 Issue 4 Pg. 522-7 (Aug 2002) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 12214866 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inhibitors
• Antineoplastic Agents
• Emulsions
• Squalene
• squalane

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Antineoplastic Agents (pharmacology)
• Cell Wall (chemistry)
• Dose-Response Relationship, Drug
• Emulsions
• Lung Neoplasms (prevention & control, secondary)
• Melanoma, Experimental
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mycobacterium bovis (chemistry)
• Neoplasm Metastasis (prevention & control)
• Neoplasm Transplantation
• Squalene (analogs & derivatives, pharmacology)
• Tumor Cells, Cultured