Enhancement of NK cytotoxicity, antimetastasis and elongation effect of survival time in B16-F10 melanoma cells by oregonin.

Abstract	We investigated the antitumor activity of oregonin, a diarylheptanoid derivative purified from Alnus hirsuta Turcz, Betulaceae. Oregonin is a potential novel immunomodulator, which augments the activation of natural killer (NK) cells, and thereby leads to a powerful antitumor activity. To evaluate the cytotoxicity of oregonin against tumor cells, we examined the effectiveness of NK cells and determined that oregonin could increase NK cell cytotoxicity. This was confirmed by MTT assay. In addition, the survival time of C57BL/6 mice were measured by inoculating B16-F10 melanoma cells to mice via intra muscular (i.m.) injection. Oregonin treatment after 10 hours of inoculation at 10 mg/kg dosage showed a significant extension of survival time by up to 51.32%, when compared to the control group. Moreover, oregonin significantly reduced the incidence of pulmonary metastasis, which may be developed from B16-F10 melanoma cells. These findings suggest that oregonin may be classified as a new and novel immunomodulator due to its potential antitumor activity.
Authors	Seong-Soo Joo, Min-Soo Kim, Won-Sik Oh, Do-Ik Lee
Journal	Archives of pharmacal research (Arch Pharm Res) Vol. 25 Issue 4 Pg. 493-9 (Aug 2002) ISSN: 0253-6269 [Print] Korea (South)
PMID	12214862 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents, Phytogenic Diarylheptanoids Tetrazolium Salts Thiazoles oregonin thiazolyl blue
Topics	Animals Antineoplastic Agents, Phytogenic (pharmacology) Cell Separation Cytotoxicity Tests, Immunologic Diarylheptanoids (pharmacology) Killer Cells, Natural (drug effects) Male Melanoma, Experimental (drug therapy, pathology) Mice Mice, Inbred C57BL Mice, Inbred ICR Neoplasm Metastasis (pathology, prevention & control) Stimulation, Chemical Survival Tetrazolium Salts Thiazoles Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!