Mayaro virus infection cycle relies on casein kinase 2 activity.

Abstract

Replication of Mayaro virus in Vero cells induces dramatic cytopathic effects and cell death. In this study, we have evaluated the role of casein kinase 2 (CK2) during Mayaro virus infection cycle. We found that CK2 was activated during the initial stages of infection ( approximately 36% after 4h). This activation was further confirmed when the enzyme was partially purified from the cellular lysate either by Mono Q 5/5Hr column or heparin-agarose column. Using this later column, we found that the elution profile of CK2 activity from infected cells was different from that obtained for control cell enzyme, suggesting a structural modification of CK2 after infection. Treatment of infected cells with a cell-permeable inhibitor of CK2, dichloro-1-(beta-D-ribofuranosyl)benzimidazole (DRB), abolished the cytopathic effect in a dose-dependent manner. Together this set of data demonstrates for the first time that CK2 activity in host cells is required in Mayaro virus infection cycle.

• Authors: Madalena M S Barroso, Carla S Lima, Mário A C Silva-Neto, Andrea T Da Poian
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 296 Issue 5 Pg. 1334-9 (Sep 06 2002) ISSN: 0006-291X [Print] United States
• PMID: 12207921 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Dichlororibofuranosylbenzimidazole
• Casein Kinase II
• Protein Serine-Threonine Kinases

Topics

• Alphavirus (growth & development, pathogenicity)
• Animals
• Casein Kinase II
• Chlorocebus aethiops
• Dichlororibofuranosylbenzimidazole (pharmacology)
• Dose-Response Relationship, Drug
• Enzyme Inhibitors (pharmacology)
• Kinetics
• Protein Serine-Threonine Kinases (antagonists & inhibitors, isolation & purification, metabolism)
• Vero Cells
• Virus Replication