The proMMP-2 activation rate in patients with chronic viral liver disease.

Abstract

BACKGROUND:

We previously showed that serum promatrix metalloproteinase-2 (proMMP-2) concentrations were increased in cirrhotic patients, reflecting the increase in liver proMMP-2 concentrations. We examined whether the increased proMMP-2 concentration reflects the biological matrix metalloproteinase-2 (MMP-2) activity.

METHODS:

We measured serum concentrations of active MMP-2 and proMMP-2, and calculated the active MMP-2/proMMP-2 ratio as an index of the proMMP-2 activation rate in chronic viral liver disease.

RESULTS:

The serum active MMP-2 concentrations were not altered in chronic liver disease, although the serum proMMP-2 concentration was markedly increased in cirrhotic patients. The active MMP-2/proMMP-2 ratio decreased with the grade of liver fibrosis, and was negatively correlated with serum levels of tissue inhibitor of metalloproteinases (TIMP)-2.

CONCLUSION:

The proMMP-2 activation rate may be inhibited by the increased TIMP-2 in liver cirrhosis (LC), resulting in the accumulation of basement membrane collagens.

• Authors: Yoshikazu Murawaki, Yujiro Ikuta, Masahiko Koda, Kinya Okamoto, Kenichi Mimura
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 324 Issue 1-2 Pg. 99-103 (Oct 2002) ISSN: 0009-8981 [Print] Netherlands
• PMID: 12204430 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 Elsevier Science B.V.

Chemical References

• Biomarkers
• Enzyme Precursors
• Gelatinases
• Metalloendopeptidases
• progelatinase
• Matrix Metalloproteinase 2

Topics

• Adult
• Biomarkers (blood)
• Chronic Disease
• Enzyme Activation
• Enzyme Precursors (blood, metabolism)
• Female
• Gelatinases (blood, metabolism)
• Humans
• Kinetics
• Liver Diseases (blood, enzymology, virology)
• Male
• Matrix Metalloproteinase 2 (blood, metabolism)
• Metalloendopeptidases (blood, metabolism)
• Middle Aged
• Virus Diseases (blood, enzymology)