Plasma kinetics of a chylomicron-like emulsion in normolipidemic obese women after a short-period weight loss by energy-restricted diet.

Abstract

Chylomicrons carry dietary fats in the bloodstream for storage in body tissues, and thus play an important role in obesity. The 2-step chylomicron metabolism consists of lipolysis by lipoprotein lipase (LPL) on vessel walls and hepatic uptake of triglyceride-depleted remnants. A triglyceride-rich emulsion that mimics chylomicrons, labeled with [9,10-(3)H]glycerol-trioleate (TG) and [1-(14)C] cholesteryl-oleate (CE) was intravenously injected into 14 obese women with body mass index between 30 and 40 kg/m(2) (age, 30 to 40 years), before and after a 2-month energy-restricted diet and into non-obese controls for determination of radioactive lipid plasma kinetics. TG kinetics evaluates lipolysis, whereas CE kinetics evaluates remnant removal. The emulsion TG fractional clearance rate (FCR, in min(-1)) was similar in obese women and their controls (0.126 +/- 0.065; controls, 0.111 +/- 0.031), but the CE-FCR was pronouncedly reduced in the obese subjects (0.028 +/- 0.014; controls, 0.070 +/- 0.009 min(-1); P <.0001). After the energy-restricted diet, TG-FCR was reduced in the obese women (0.075 +/- 0.044 min(-1); P <.05), but CE-FCR was unchanged (0.032 +/- 0.025 min(-1)). Therefore, the lipolysis of the chylomicron-like emulsion is normal in obese women, but remnant removal from the plasma is diminished. After active weight loss by an energy-restricted diet, the remnant removal was unchanged but lipolysis was diminished, possibly due to adaptative changes in LPL activity.

• Authors: M R M Oliveira, R C Maranhão
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 51 Issue 9 Pg. 1097-103 (Sep 2002) ISSN: 0026-0495 [Print] United States
• PMID: 12200752 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002, Elsevier Science (USA). All rights reserved.

Chemical References

• Apolipoproteins
• Blood Glucose
• Chylomicrons
• Dietary Carbohydrates
• Emulsions
• Lipids

Topics

• Adult
• Anthropometry
• Apolipoproteins (blood)
• Blood Glucose (analysis)
• Chylomicrons (blood)
• Diet
• Diet, Fat-Restricted
• Dietary Carbohydrates (administration & dosage)
• Emulsions
• Female
• Humans
• Kinetics
• Lipids (blood)
• Obesity (blood, diet therapy, pathology)
• Reference Values
• Time Factors
• Weight Loss