Abstract | BACKGROUND: We previously reported that the amount of annexin IV significantly increased in the postmortem brains of alcoholics compared with controls. To investigate further whether ethanol directly affects cellular expression of annexins in a cell, we used cell lines and compared the amounts of annexins between ethanol-exposed and control cells. METHODS: RESULTS: Expressive augmentation of annexin IV was shown in both C6 and A549 cells after a 5-hr exposure to 200 mM of ethanol, whereas amounts of annexins I and V were not changed. Both the mitochondrial dehydrogenase and caspase 3 activities were altered under the same conditions in C6 cells, indicating the induction of cell damage (apoptosis), whereas both of these enzyme activities were unchanged in A549 cells under the same conditions. CONCLUSIONS:
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Authors | Hitoshi Sohma, Hiroko Ohkawa, Eri Hashimoto, Rie Sakai, Toshikazu Saito |
Journal | Alcoholism, clinical and experimental research
(Alcohol Clin Exp Res)
Vol. 26
Issue 8 Suppl
Pg. 44S-48S
(Aug 2002)
ISSN: 0145-6008 [Print] England |
PMID | 12198374
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Annexin A4
- Phospholipids
- Ethanol
- Oxidoreductases
- CASP3 protein, human
- Casp3 protein, rat
- Caspase 3
- Caspases
- Calcium
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Topics |
- Adenocarcinoma
- Alcoholism
(pathology)
- Animals
- Annexin A4
(metabolism)
- Apoptosis
(drug effects)
- Blotting, Western
- Brain
(pathology)
- Brain Neoplasms
- Calcium
(metabolism)
- Caspase 3
- Caspases
(metabolism)
- Ethanol
(toxicity)
- Glioma
- Humans
- Mitochondria
(pathology)
- Neurons
(drug effects, pathology)
- Oxidoreductases
(metabolism)
- Phospholipids
(metabolism)
- Rats
- Tumor Cells, Cultured
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