Abstract |
D-RNAi ( Messenger RNA- antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4(+) T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99% endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.
|
Authors | S L Lin, S Sukasweang, C M Chuong, S Rasheed, S Y Ying |
Journal | Current cancer drug targets
(Curr Cancer Drug Targets)
Vol. 1
Issue 3
Pg. 241-7
(Nov 2001)
ISSN: 1568-0096 [Print] Netherlands |
PMID | 12188882
(Publication Type: Journal Article, Review)
|
Chemical References |
- DNA, Antisense
- DNA, Complementary
- RNA, Messenger
- RNA, Small Interfering
- RNA, Untranslated
|
Topics |
- DNA, Antisense
(pharmacology)
- DNA, Complementary
(genetics, metabolism)
- Gene Silencing
- Humans
- Neoplasms
(genetics, therapy)
- RNA Processing, Post-Transcriptional
(genetics, physiology)
- RNA, Messenger
(genetics, metabolism)
- RNA, Small Interfering
- RNA, Untranslated
(genetics, metabolism)
- Transcription, Genetic
|