Neuropeptide F and its expression in the yellow fever mosquito, Aedes aegypti.

Abstract

A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal.

• Authors: Dawn M Stanek, Jan Pohl, Joe W Crim, Mark R Brown
• Journal: Peptides (Peptides) Vol. 23 Issue 8 Pg. 1367-78 (Aug 2002) ISSN: 0196-9781 [Print] United States
• PMID: 12182937 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 2002 Elsevier Science Inc.

Chemical References

• Neuropeptides
• neuropeptide F

Topics

• Aedes (genetics)
• Amino Acid Sequence
• Animals
• Base Sequence
• Blotting, Northern
• Conserved Sequence (genetics)
• Disease Vectors
• Female
• Immunohistochemistry
• Male
• Molecular Sequence Data
• Neuropeptides (biosynthesis, blood, genetics, isolation & purification)
• Yellow Fever (transmission)