Mechanisms of actio of poly-plat, a novel platinum antineoplastic agent.

Abstract

Action of Cisplatin (cis-dichlorodiammine platinum II) and Poly-Plat [poly-[(trans-1,2-diaminocyclohexane) pla-tinum]-carboxyamylose] on tumor cells was examined using human fibrosarcoma (HT1080) and Walker rat carcinoma (WRC-256) in culture. These cells were treated with Poly-Plat (10 microg/ml) or Cisplatin (10 mg/ml) for 2-5 days. Peritoneal macrophages were treated with Cisplatin (10 microg/ml) or Poly-Plat (10 microg/ml) for 2 hours and allowed to grow in normal medium for 48 hours. Supernatants from treated macrophages were collected at 0, 4, 12, 24 and 48 hours and IL-2 levels were examined using ELISA technique. The cytotoxicity of these supernatants was examined using HT1080 and WRC-256 cells. Apoptotic assays were used to determine the cytotoxic effects of drugs and macrophage supernatants on tumor cells. These results demonstrate the ability of Poly-Plat to arrest the growth of tumor cells and activate macrophages to induce apoptosis in the cell lines tested.

• Authors: Dale J Telgenhoff, Surinder K Aggarwal
• Journal: Anticancer research (Anticancer Res) 2002 Jul-Aug Vol. 22 Issue 4 Pg. 2167-72 ISSN: 0250-7005 [Print] Greece
• PMID: 12174899 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Interleukin-2
• Organoplatinum Compounds
• poly(trans-1,2-diaminocyclohexane)platinum-carboxyamylose
• Cisplatin

Topics

• Antineoplastic Agents (toxicity)
• Apoptosis (drug effects)
• Cell Survival (drug effects)
• Cisplatin (toxicity)
• Fibrosarcoma (pathology)
• Humans
• Interleukin-2 (analysis)
• Macrophages (drug effects, immunology)
• Mitosis (drug effects)
• Organoplatinum Compounds (toxicity)
• Tumor Cells, Cultured