Abstract |
We studied the effect of momordin I, a compound purified from a plant, Ampelopsis japonica, on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Momordin I was cytotoxic to HL-60 cells with an IC50 of 19.0 microg/ml. The antiproliferative effects of momordin I appear to be attributable to its induction of apoptotic cell death, as momordin I induced nuclear morphology changes and internucleosomal DNA fragmentation and it increased the proportion of hypodiploid cells. Momordin I treatment also gradually decreased the expression of.the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic protein Bax. In addition, momordin I treatment increased the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase. In this study we showed that momordin I induced apoptosis of HL-60 cells by reduction of the Bcl-2:Bax ratio and by activation of caspase-3. These results provide important information towards understanding the mechanism by which momordin I induces apoptosis.
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Authors | Jeong Hee Kim, Eun-Mi Ju, Dug Keun Lee, Hyun Jin Hwang |
Journal | Anticancer research
(Anticancer Res)
2002 May-Jun
Vol. 22
Issue 3
Pg. 1885-9
ISSN: 0250-7005 [Print] Greece |
PMID | 12168888
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BAX protein, human
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-bcl-2
- Saponins
- bcl-2-Associated X Protein
- momordin I (Oleanolic Acid)
- Oleanolic Acid
- Poly(ADP-ribose) Polymerases
- CASP3 protein, human
- Caspase 3
- Caspases
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Topics |
- Apoptosis
(drug effects)
- Caspase 3
- Caspases
(metabolism)
- Cell Division
(drug effects)
- Enzyme Activation
(drug effects)
- HL-60 Cells
(cytology, drug effects, metabolism)
- Humans
- Oleanolic Acid
(analogs & derivatives)
- Poly(ADP-ribose) Polymerases
(metabolism)
- Proto-Oncogene Proteins
(biosynthesis)
- Proto-Oncogene Proteins c-bcl-2
(biosynthesis)
- Saponins
(pharmacology)
- bcl-2-Associated X Protein
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