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Induction of apoptosis by momordin I in promyelocytic leukemia (HL-60) cells.

Abstract
We studied the effect of momordin I, a compound purified from a plant, Ampelopsis japonica, on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Momordin I was cytotoxic to HL-60 cells with an IC50 of 19.0 microg/ml. The antiproliferative effects of momordin I appear to be attributable to its induction of apoptotic cell death, as momordin I induced nuclear morphology changes and internucleosomal DNA fragmentation and it increased the proportion of hypodiploid cells. Momordin I treatment also gradually decreased the expression of.the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic protein Bax. In addition, momordin I treatment increased the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase. In this study we showed that momordin I induced apoptosis of HL-60 cells by reduction of the Bcl-2:Bax ratio and by activation of caspase-3. These results provide important information towards understanding the mechanism by which momordin I induces apoptosis.
AuthorsJeong Hee Kim, Eun-Mi Ju, Dug Keun Lee, Hyun Jin Hwang
JournalAnticancer research (Anticancer Res) 2002 May-Jun Vol. 22 Issue 3 Pg. 1885-9 ISSN: 0250-7005 [Print] Greece
PMID12168888 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • BAX protein, human
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Saponins
  • bcl-2-Associated X Protein
  • momordin I (Oleanolic Acid)
  • Oleanolic Acid
  • Poly(ADP-ribose) Polymerases
  • CASP3 protein, human
  • Caspase 3
  • Caspases
Topics
  • Apoptosis (drug effects)
  • Caspase 3
  • Caspases (metabolism)
  • Cell Division (drug effects)
  • Enzyme Activation (drug effects)
  • HL-60 Cells (cytology, drug effects, metabolism)
  • Humans
  • Oleanolic Acid (analogs & derivatives)
  • Poly(ADP-ribose) Polymerases (metabolism)
  • Proto-Oncogene Proteins (biosynthesis)
  • Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
  • Saponins (pharmacology)
  • bcl-2-Associated X Protein

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