Abstract | OBJECTIVE: METHODS: Models of incomplete brain ischemia were induced by clipping common carotid artery. A total of 46 aged SD rats were divided into 8 groups: normal control group (Group A, n=5), sham-operation group (Group B, n=5), reperfusion 1, 6, 12, 24, 48, and 96 hours groups after brain ischemia for 30 minutes (Group C, D, E, F, G, and H, n=6/group). The expression of nNOS was examined by immunohistochemistry and neuronal ultrastructural changes were observed by the transmission electron microscopy (TEM) at different time points after reperfusion. RESULTS: Immunohistochemistry showed that nNOS expression in the hippocampal neurons was high in Group E, low expression in Group D, moderate expression in Group F and G. There was nearly no expression of nNOS in Group A, B, C, and H. Ultrastructure of hippocampal neurons was damaged more severely in reperfusion over 24 hours groups. CONCLUSIONS:
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Authors | Chuanhong Yang, Huangwen Lai, Chunlie Zhan, Yuhua Xiao, Wenling Zheng |
Journal | Chinese journal of traumatology = Zhonghua chuang shang za zhi
(Chin J Traumatol)
Vol. 5
Issue 4
Pg. 232-6
(Aug 2002)
ISSN: 1008-1275 [Print] China |
PMID | 12162902
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial)
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Chemical References |
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Topics |
- Animals
- Apoptosis
- Brain Ischemia
(enzymology)
- Female
- Hippocampus
(enzymology, pathology)
- Immunohistochemistry
- Male
- Microscopy, Electron
- Neurons
(enzymology)
- Nitric Oxide Synthase
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(enzymology)
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