Sialyl Lewis X-dependent lung colonization of B16 melanoma cells through a selectin-like endothelial receptor distinct from E- or P-selectin.

Abstract

Endothelial carbohydrate binding proteins, E- and P-selectins, are thought to mediate sialyl Lewis A/X-dependent hematogenous cancer metastasis. We tested this hypothesis using sialyl Lewis X-dependent B16 melanoma lung targeting and its inhibition with selectin ligand mimicry peptide, IELLQAR. In E/P-selectin doubly deficient mutant mice, sialyl Lewis X-expressing B16 melanoma cells colonized the lung, and IELLQAR inhibited this colonization. However, tumors grown in E/P-selectin-deficient mice were significantly smaller than those grown in wild-type mice. These results indicate that the IELLQAR peptide receptor expressed in the lung vasculature plays a major role in sialyl Lewis X-dependent cancer cells targeting to the lung.

• Authors: Jianing Zhang, Jun Nakayama, Chikara Ohyama, Masami Suzuki, Atsushi Suzuki, Minoru Fukuda, Michiko N Fukuda
• Journal: Cancer research (Cancer Res) Vol. 62 Issue 15 Pg. 4194-8 (Aug 01 2002) ISSN: 0008-5472 [Print] United States
• PMID: 12154017 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• E-Selectin
• Oligopeptides
• Oligosaccharides
• P-Selectin
• Receptors, Peptide
• Sialyl Lewis X Antigen
• isoleucyl-glutamyl-leucyl-leucyl-glutaminyl-alanyl-arginine

Topics

• Animals
• E-Selectin (biosynthesis, physiology)
• Lung (metabolism)
• Lung Neoplasms (blood supply, metabolism, prevention & control, secondary)
• Melanoma, Experimental (blood supply, prevention & control, secondary)
• Mice
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Molecular Mimicry
• Neovascularization, Pathologic (metabolism)
• Oligopeptides (metabolism, pharmacology)
• Oligosaccharides (antagonists & inhibitors, physiology)
• P-Selectin (biosynthesis, physiology)
• Receptors, Peptide (biosynthesis, metabolism, physiology)
• Sialyl Lewis X Antigen