Abstract |
The effects of insect cell culture medium supplementation with FBS were investigated. BSA was found to be the factor responsible for the increased baculovirus infection rate of FBS-supplemented cultures in a concentration-dependent form up to 25 g L(-)(1). Lower rates of baculovirus binding to cells were observed with FBS- and BSA-supplemented cultures compared with infections carried out in serum-free media. Virus attachment constants were found to depend on medium matrix composition. An efficiency factor dependent on the medium matrix composition was introduced to account for these effects, and a mathematical model was developed to describe the virus-cell interactions. It was shown that BSA acts by minimizing the nonspecific virus binding leading to an increased cell infection rate. Cell specific Porcine parvovirusvirus-like particles (PPV-VLPs) expression was unaffected by medium supplementation pointing out that BSA and/or FBS affects mainly the initial phase of the baculovirus infection cycle. Implications for process definition are discussed.
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Authors | Luis Maranga, Ana S Coroadinha, Manuel J T Carrondo |
Journal | Biotechnology progress
(Biotechnol Prog)
2002 Jul-Aug
Vol. 18
Issue 4
Pg. 855-61
ISSN: 8756-7938 [Print] United States |
PMID | 12153321
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media
- Serum Albumin, Bovine
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Topics |
- Adsorption
(drug effects)
- Animals
- Baculoviridae
(drug effects, genetics, physiology)
- Cattle
- Cell Culture Techniques
(methods)
- Culture Media
(chemistry)
- Fetal Blood
(chemistry)
- Gene Expression
- Kinetics
- Models, Biological
- Molecular Biology
(methods)
- Serum Albumin, Bovine
(isolation & purification, pharmacology)
- Spodoptera
(cytology, drug effects, virology)
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