Identification of downstream genes up-regulated by the tumor necrosis factor family member TALL-1.

Abstract

TALL-1 is a member of the tumor necrosis factor family that binds to BCMA, TACI, and BAFF-R, three receptors mostly expressed by mature B lymphocytes. Previous studies have shown that the TALL-1 signaling is critically involved in B cell proliferation, maturation, and progression of lupus-like, autoimmune diseases. In this report, we performed cDNA subtractive hybridization experiments to identify downstream genes up-regulated by TALL-1. These experiments indicated that 10 genes, including interleukin (IL)-10, lymphocyte activation gene-1 (LAG-1), GCP-2, PBEF, ferritin, PIM-2, TFG, CD27 ligand, DUSP5, and archain, were up-regulated at the mRNA level by TALL-1 stimulation in B lymphoma RPMI-8226 cells and/or primary B lymphocytes. We also demonstrated that TALL-1 activated transcription of IL-10 and LAG-1 in a nuclear factor-kappaB-dependent manner in reporter gene assays. Moreover, our findings indicated BAFF-R, but not TACI, could dramatically up-regulate IL-10 secretion by RPMI-8226 cells. The identification of TALL-1-up-regulated genes will help explain the mechanisms of TALL-1-triggered biological and pathological effects and to identify molecular targets for intervention of lupus-like autoimmune diseases.

• Authors: Liang-Guo Xu, Min Wu, Jiancheng Hu, Zhonghe Zhai, Hong-Bing Shu
• Journal: Journal of leukocyte biology (J Leukoc Biol) Vol. 72 Issue 2 Pg. 410-6 (Aug 2002) ISSN: 0741-5400 [Print] United States
• PMID: 12149433 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• B-Cell Activating Factor
• B-Cell Activation Factor Receptor
• B-Cell Maturation Antigen
• DNA, Complementary
• Membrane Proteins
• NF-kappa B
• Neoplasm Proteins
• RNA, Messenger
• RNA, Neoplasm
• Receptors, Tumor Necrosis Factor
• Recombinant Fusion Proteins
• TNFRSF13B protein, human
• TNFRSF13C protein, human
• TNFRSF17 protein, human
• TNFSF13B protein, human
• Transmembrane Activator and CAML Interactor Protein
• Tumor Necrosis Factor-alpha

Topics

• B-Cell Activating Factor
• B-Cell Activation Factor Receptor
• B-Cell Maturation Antigen
• B-Lymphocytes (metabolism)
• DNA, Complementary (genetics)
• Gene Expression Profiling
• Gene Expression Regulation (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Genes, Reporter
• Humans
• Lymphoma, B-Cell (metabolism, pathology)
• Membrane Proteins (pharmacology, physiology)
• NF-kappa B (metabolism)
• Neoplasm Proteins (biosynthesis, genetics, physiology)
• Neoplastic Stem Cells (metabolism)
• RNA, Messenger (biosynthesis)
• RNA, Neoplasm (biosynthesis)
• Receptors, Tumor Necrosis Factor (drug effects, physiology)
• Recombinant Fusion Proteins (biosynthesis, genetics, pharmacology)
• Signal Transduction
• Subtraction Technique
• Transcription, Genetic (drug effects)
• Transmembrane Activator and CAML Interactor Protein
• Tumor Cells, Cultured (drug effects, metabolism)
• Tumor Necrosis Factor-alpha (pharmacology, physiology)