Abstract | INTRODUCTION: AIM: METHODOLOGY:
Acute pancreatitis was induced by intraperitoneal infusion of cerulein in rats. Emodin was administered intravenously and Sandostatin was administered subcutaneously at the time of induction of pancreatitis and 24, 48, and 72 hours afterward. Rats were killed at 6, 24, 48, 72, and 96 hours after the operation. The mRNA expression of TGFbeta1 and EGF were evaluated by reverse transcription polymerase chain reaction, and pancreatic tissue DNA synthesis was measured by the 3H-thymidine incorporation method in vitro. Total protein content was detected by Lowry's method. RESULTS: The serum amylase level was decreased significantly in the emodin-treated and Sandostatin-treated groups in comparison with the nontreated group. Pancreatic tissue DNA synthesis was significantly decreased at 72 hours after the induction of pancreatitis, and a marked increase was observed at 96 hours after treatment with emodin and Sandostatin. Within 48 hours of the induction of pancreatitis, the total protein content in pancreatic tissue declined, but there was a remarkable increase in the emodin-treated group at 96 hours and Sandostatin-treated group at 48 hours. Expression of TGFbeta1 mRNA and EGF mRNA were undetectable in normal pancreas and the nontreated group at 6 hours but was observed from 24 hours to 96 hours after the induction of pancreatitis and reached its maximum at 72 hours. TGFbeta1 mRNA could be detected 6 hours after treatment with emodin and Sandostatin, and its expression was significantly higher in the emodin-treated and Sandostatin-treated groups than in the nontreated group at 24 and 48 hours. The expression of EGF mRNA was significantly higher in the emodin-treated and Sandostatin-treated group than in the nontreated group at 48 hours. CONCLUSION: It was concluded that mechanisms of the Chinese herb emodin and somatostatin analogs in the management of acute pancreatitis in rats might be ascribed to the upregulation of TGFbeta1 and EGF gene expression, which subsequently increases DNA synthesis and protein content and thus accelerates pancreatic repair and regeneration.
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Authors | Zihua Gong, Yaozog Yuan, Kaixian Lou, Shuiping Tu, Zukang Zhai, Jiayu Xu |
Journal | Pancreas
(Pancreas)
Vol. 25
Issue 2
Pg. 154-60
(Aug 2002)
ISSN: 1536-4828 [Electronic] United States |
PMID | 12142738
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- Enzyme Inhibitors
- Hormones
- Proteins
- RNA, Messenger
- Tgfb1 protein, rat
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
- Epidermal Growth Factor
- DNA
- Amylases
- Emodin
- Octreotide
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Topics |
- Acute Disease
- Amylases
(blood, drug effects)
- Animals
- DNA
(biosynthesis, drug effects)
- Drugs, Chinese Herbal
(pharmacology)
- Emodin
(pharmacology)
- Enzyme Inhibitors
(pharmacology)
- Epidermal Growth Factor
(genetics)
- Gene Expression Regulation
(drug effects)
- Hormones
(pharmacology)
- Male
- Microscopy, Electron
- Octreotide
(pharmacology)
- Pancreas
(drug effects, physiopathology, ultrastructure)
- Pancreatitis
(drug therapy, genetics, metabolism)
- Protein Biosynthesis
- Proteins
(drug effects)
- RNA, Messenger
(drug effects, genetics, metabolism)
- Rats
- Rats, Wistar
- Regeneration
(drug effects)
- Time Factors
- Transforming Growth Factor beta
(genetics)
- Transforming Growth Factor beta1
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