Expression of type I interferon receptor in liver and peripheral blood mononuclear cells in chronic hepatitis C patients.

Abstract

To assess whether peripheral blood mononuclear cells (PBMCs) can be substituted for liver tissue in monitoring the hepatic expression of interferon receptors (IFNAR1 and/or IFNAR2) in chronic hepatitis C, we quantified the mRNA of both subunits, using the competitive polymerase chain reaction, in the liver specimens from 37 chronic hepatitis C patients and in PBMCs from 29 of 37 patients. PBMCs from 10 healthy subjects were also quantified for both subunits. Hepatic expression of IFNAR1 and IFNAR2 were more frequent than PBMCs: IFNAR1, 23/27 (85%) vs 7/19 (37%), P = 0.0021, IFNAR2, 34/37 (92%) vs 19/29 (66%), P = 0.0182. Neither subunits was detected in PBMCs from control subjects. The expression level of IFNAR2 in the liver was related to that in PBMCs (r = 0.613, P = 0.0052). These results suggest that hepatitis C virus infection may up-regulate the expression of the type I interferon receptor and that the measurement of IFNAR2 expression in PBMCs may be useful for monitoring its expression in liver.

• Authors: Yuhki Yamaguchi, Keisuke Hino, Daisuke Fujiwara, Fenyu Ren, Yoshiharu Katoh, Yasuhiro Satoh, Kiwamu Okita
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 47 Issue 7 Pg. 1611-7 (Jul 2002) ISSN: 0163-2116 [Print] United States
• PMID: 12141824 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Interferon

Topics

• Adult
• Aged
• Female
• Hepatitis C, Chronic (metabolism)
• Humans
• Liver (metabolism)
• Male
• Middle Aged
• Neutrophils (metabolism)
• Polymerase Chain Reaction
• Receptors, Interferon (metabolism)
• Sensitivity and Specificity