Deletions of SKY1 or PTK2 in the Saccharomyces cerevisiae trk1Deltatrk2Delta mutant cells exert dual effect on ion homeostasis.

Abstract	Sky1p and Ptk2p are protein kinases that regulate ion transport across the plasma membrane of Saccharomyces cerevisiae. We show here that deletion of SKY1 or PTK2 in trk1,2Delta cells increase spermine tolerance, implying Trk1,2p independent activity. Unexpectedly, trk1,2Deltasky1Delta and trk1,2Deltaptk2Delta cells display hypersensitivity to LiCl. These cells also show increased tolerance to low pH and improved growth in low K(+), as demonstrated for deletion of PMP3 in trk1,2Delta cells. We show that Sky1p and Pmp3p act in different pathways. Hypersensitivity to LiCl and improved growth in low K(+) are partly dependent on the Nha1p and Kha1p antiporters and on the Tok1p channel. Finally, Dhh1p, a RNA helicase was demonstrated to improve growth of trk1,2Deltasky1Delta cells in low K(+). Overexpression of Dhh1p improves the ability of trk1,2Delta cells to grow in low K(+) while dhh1Delta cells are sensitive to spermine and salt ions. A model that integrates these results to explain the mechanism of ion transport across the plasma membrane is proposed.
Authors	Omri Erez, Chaim Kahana
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 295 Issue 5 Pg. 1142-9 (Aug 02 2002) ISSN: 0006-291X [Print] United States
PMID	12135613 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Carrier Proteins Cation Transport Proteins Ions KHA1 protein, S cerevisiae Membrane Proteins NHA1 protein, S cerevisiae PMP3 protein, S cerevisiae Potassium Channels Potassium-Hydrogen Antiporters Proteolipids RNA-Binding Proteins Saccharomyces cerevisiae Proteins Sodium-Hydrogen Exchangers TOK1 protein, S cerevisiae TRK2 protein, S cerevisiae TRK1 protein, S cerevisiae SKY1 protein, S cerevisiae Protein-Tyrosine Kinases Focal Adhesion Protein-Tyrosine Kinases Protein Serine-Threonine Kinases DHH1 protein, S cerevisiae DEAD-box RNA Helicases RNA Helicases Potassium
Topics	Carrier Proteins (metabolism) Cation Transport Proteins DEAD-box RNA Helicases Focal Adhesion Protein-Tyrosine Kinases Gene Deletion Homeostasis (physiology) Ions (metabolism) Membrane Proteins (deficiency, metabolism) Phenotype Potassium (metabolism) Potassium Channels (metabolism) Potassium-Hydrogen Antiporters (metabolism) Protein Serine-Threonine Kinases (deficiency, genetics, metabolism) Protein-Tyrosine Kinases (deficiency, genetics, metabolism) Proteolipids (metabolism) RNA Helicases (physiology) RNA-Binding Proteins Saccharomyces cerevisiae (genetics, metabolism) Saccharomyces cerevisiae Proteins (metabolism) Sodium-Hydrogen Exchangers (metabolism)

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