Cytotoxic and antimutagenic stilbenes from seeds of Paeonia lactiflora.

Abstract

Cytotoxic and antimutagenic effects of a novel cis-epsilon-viniferin and five known stilbenes, transresveratrol, trans-epsilon-viniferin, gnetin H, suffruticosols A and B, isolated from the seeds of Paeonia lactiflora Pall. (Paeoniaceae) were determined against five different cancer cell lines, and mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100, respectively. Six stilbenes showed cytotoxic activity in a dose-dependent manner, and especially did potent cytotoxic activity against C6 (mouse glioma) cancer cell with IC50 values ranging from 8.2 to 20.5 microg/ml. trans-Resveratrol showed significant cytotoxic activity against HepG2 (liver hepatoma) and HT-29 (colon) human cancer cell lines with IC50 values of 11.8 and 25.2 g/ml, respectively. In contrast, trans-epsilon-viniferin and cis--viniferin, and gnetin H exhibited marked cytotoxic activity against Hela (cervicse) and MCF-7 (breast) human cancer cell lines with IC50 values of 20.4, 21.5, and 12.9 microg/ml, respectively. However, suffruticosol A and B had less cytotoxic effect against all cancer cells except C6. Meanwhile, six stilbenes exerted antimutagenic activity in a dose-dependent fashion. Of them, trans-resveratrol exhibited the strongest antimutagenic effect against MNNG with IC50 value of 27.0 microg/plate, while other five resveratrol oligomers also did moderate antimutagenic activity with IC50 values ranging from 31.7 to 35.2 microg/plate.

• Authors: Hyo Jin Kim, Eun Ju Chang, Song Ja Bae, Sun Mi Shim, Heui Dong Park, Chang Ho Rhee, Jun Hong Park, Sang Won Choi
• Journal: Archives of pharmacal research (Arch Pharm Res) Vol. 25 Issue 3 Pg. 293-9 (Jun 2002) ISSN: 0253-6269 [Print] Korea (South)
• PMID: 12135100 (Publication Type: Journal Article)

Chemical References

• Antimutagenic Agents
• Antineoplastic Agents, Phytogenic
• Stilbenes

Topics

• Animals
• Antimutagenic Agents (chemistry, pharmacology)
• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Chromatography, Thin Layer
• Drug Screening Assays, Antitumor
• Humans
• In Vitro Techniques
• Magnetic Resonance Spectroscopy
• Paeonia (chemistry)
• Rats
• Salmonella typhimurium (drug effects)
• Seeds (chemistry)
• Spectrometry, Mass, Fast Atom Bombardment
• Spectrophotometry, Infrared
• Spectrophotometry, Ultraviolet
• Spectroscopy, Fourier Transform Infrared
• Stilbenes (chemistry, pharmacology)