Prognostic value of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in bladder carcinoma.

Abstract

OBJECTIVES:

To detect the level of matrix metalloproteinase-2 (MMP-2) mRNA and the tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in bladder transitional cell carcinoma (BTCC), and to estimate the prognosis for bladder tumor based on the quality and quantity of MMP-2 and TIMP-2 mRNA.

METHODS:

Thirty-five samples of human BTCC and 15 normal fresh bladder tissues were studied by RT-PCR analysis followed by computer-assisted image analysis.

RESULTS:

The level of the MMP-2 mRNA in BTCC was significantly increased compared with that in normal bladder epithelium. The positive rates of MMP-2 and TIMP-2 mRNA were 71.4% and 65.7% in BTCC, and 66.7% and 60.0% in the normal bladder wall. The expression intensity of the MMP-2 mRNA by image analysis tended to increase with tumor grading and staging, which showed statistical significance. Similarly, the MMP-2 to TIMP-2 ratio also showed statistically significant difference between normal bladder tissue and bladder carcinoma (P < 0.01).

CONCLUSIONS:

A high level of the MMP-2 mRNA exists in BTCC, which may function to damage collagen IV inside the basement membrane and the extracellular basement of the bladder. The level of the MMP-2 mRNA is proportional to BTCC grading and staging, which may have prognostic value. The MMP-2 to TIMP-2 ratio may play a more significant role in the determination of the aggressiveness and prognosis of bladder tumor.

• Authors: Kexin Xu, Shukun Hou, Zhijun Du
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 115 Issue 5 Pg. 743-5 (May 2002) ISSN: 0366-6999 [Print] China
• PMID: 12133547 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Tissue Inhibitor of Metalloproteinase-2
• Matrix Metalloproteinase 2

Topics

• Adult
• Aged
• Aged, 80 and over
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Matrix Metalloproteinase 2 (genetics)
• Middle Aged
• Neoplasm Staging
• Predictive Value of Tests
• Prognosis
• RNA, Messenger (genetics, metabolism)
• Tissue Inhibitor of Metalloproteinase-2 (genetics)
• Urinary Bladder (metabolism, pathology)
• Urinary Bladder Neoplasms (genetics, pathology)