Fructose-1,6-diphosphate attenuates acute lung injury induced by ischemia-reperfusion in rats.

Abstract

OBJECTIVE:

To determine whether fructose-1,6-diphosphate (FDP) pretreatment can attenuate acute lung injury induced by ischemia-reperfusion in our isolated lung model in rats.

DESIGN:

Randomized, controlled study.

SETTING:

Animal care facility procedure room.

SUBJECTS:

Twenty-four adult male Sprague-Dawley rats each weighing 250-350 g.

INTERVENTIONS:

Typical acute lung injury in rats was induced successfully by 10 mins of hypoxia followed by 75 mins of ischemia and 50 mins of reperfusion. Ischemia-reperfusion significantly increased microvascular permeability as measured by the capillary filtration coefficient, lung weight gain, lung weight to body weight ratio, pulmonary arterial pressure, and protein concentration of bronchoalveolar lav-age fluid.

MEASUREMENTS AND MAIN RESULTS:

Pretreatment with FDP significantly attenuated the acute lung injury induced by ischemia-reperfusion as shown by a significant decrease in all of the assessed variables (p <.05 p <.001). The protective effect of FDP was nearly undetectable when promazine (an ecto-adenosine 5-triphosphatase inhibitor) was added before FDP pretreatment.

CONCLUSIONS:

Pretreatment with FDP significantly ameliorates acute lung injury induced by ischemia-reperfusion in rats.

• Authors: Shi-Jye Chu, Deh-Ming Chang, David Wang, Ying-Hsin Chen, Chin-Wang Hsu, Kang Hsu
• Journal: Critical care medicine (Crit Care Med) Vol. 30 Issue 7 Pg. 1605-9 (Jul 2002) ISSN: 0090-3493 [Print] United States
• PMID: 12130986 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fructosediphosphates
• Immunologic Factors
• fructose-1,6-diphosphate
• Promazine

Topics

• Animals
• Blood Pressure
• Capillary Permeability
• Fructosediphosphates (antagonists & inhibitors, therapeutic use)
• Immunologic Factors (therapeutic use)
• In Vitro Techniques
• Male
• Organ Size
• Promazine (pharmacology)
• Pulmonary Artery
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury
• Respiratory Distress Syndrome (drug therapy, etiology, pathology)