N-ethyl-N-hydroxyethylnitrosamine (EHEN)-induced renal and hepatocarcinogenesis in the tumor suppressor Tsc2 transgenic rat.

Abstract	Hereditary renal carcinomas (RCs) develop in Tsc2 gene mutant (Eker) rats around the age of 1 year. We previously reported that Tsc2 mutations were detected in chemically (N-ethyl-N-hydroxyethylnitrosamine (EHEN) and diethylnitrosamine)-induced non-Eker rat RCs, suggesting an involvement of Tsc2 alteration in rat RC development. In this study, we evaluated the effect of extra copies of the Tsc2 gene on renal and hepatocarcinogenesis that was induced by EHEN in vivo. The incidence of RCs in non-transgenic rats (2/17) is slightly higher than in transgenic rats (0/32), although it is statistically not significant. These results suggest the presence of other target RC gene(s) in chemically (EHEN)-induced renal carcinogenesis. We observed no difference in the numbers and areas of the hepatic glutathione S-transferase placental type positive foci.
Authors	Nobuo Satake, Makoto Miyagawa, Junko Sakurai, Hiroaki Mitani, Toshiyuki Kobayashi, Hiroshi Tamura, Okio Hino
Journal	Cancer letters (Cancer Lett) Vol. 184 Issue 2 Pg. 157-63 (Oct 28 2002) ISSN: 0304-3835 [Print] Ireland
PMID	12127687 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Carcinogens Repressor Proteins TSC2 protein, human Tsc2 protein, rat Tuberous Sclerosis Complex 2 Protein Tumor Suppressor Proteins N-ethyl-N-hydroxyethylnitrosamine Diethylnitrosamine
Topics	Animals Animals, Genetically Modified Carcinogens (toxicity) Diethylnitrosamine (analogs & derivatives, toxicity) Female Gene Expression Regulation Genes, Tumor Suppressor Humans Kidney Neoplasms (chemically induced, genetics, pathology, prevention & control) Liver Neoplasms (chemically induced, genetics, pathology, prevention & control) Male Rats Rats, Wistar Repressor Proteins (genetics) Sex Characteristics Tuberous Sclerosis Complex 2 Protein Tumor Suppressor Proteins

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