Abstract | OBJECTIVE: METHODS: Establish the rat model of hind limb ischemia / reperfusion. One hundred and two rats were divided into 5 groups: sham operation group (Group I n = 6), ischemia group (GroupII n = 6), ischemia / reperfusion injury group (Group III n = 30), normal saline treatment group (Group IV n = 30), superoxide dismutase treatment group (Group V n = 30). Flow cytometric analysis and immunohistochemistry were used to assess the expression of CD11b/CD18 on the leukocytes, ICAM-1 in skeletal muscle at the end of ischemia and 1h, 2h, 4h,8h,12h after reperfusion. The changes of MDA in the plasma,MPO in the skeletal muscle and the tissue morphology were studied too. RESULTS: In group III,the expression of CD11b/CD18, ICAM-1,MDA,MPO were increased evidently. The longer time of reperfusion,the more serious the skeletal muscle's injury was. In group V, the changes were ameliorated as compared with group III (P < 0.05), group IV has no difference with group III. CONCLUSION:
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Authors | Songcen Lu, Xintao Wang, Lichen Wen, Zhu Han, Tianzun Tao, Dehong Guan |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 82
Issue 12
Pg. 840-3
(Jun 25 2002)
ISSN: 0376-2491 [Print] China |
PMID | 12126535
(Publication Type: Journal Article)
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Chemical References |
- CD18 Antigens
- Macrophage-1 Antigen
- Intercellular Adhesion Molecule-1
- Superoxide Dismutase
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Topics |
- Animals
- CD18 Antigens
(biosynthesis)
- Intercellular Adhesion Molecule-1
(biosynthesis)
- Macrophage-1 Antigen
(biosynthesis)
- Male
- Muscle, Skeletal
(injuries, metabolism)
- Rats
- Rats, Wistar
- Reperfusion Injury
(metabolism)
- Superoxide Dismutase
(metabolism)
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