Abstract | OBJECTIVE: METHODS: Thirty specimens of esophageal squamous epithelial cancer were resected during operation. A piece of cancer tissue and a piece of juxtacancerous tissue were taken from each specimen. Fourteen specimens of preinvasive esophageal carcer were obtained by esophagoscopy. Reverse transcription polymerase chain reaction (RT-PCR), single-strand conformation polymorphism (SSCP) and sequence analysis were used to examine the DNA polymerase beta genes. DNASIS and OMIGA softwares were used for sequencing. RESULTS: Obvious mutation was detected by SSCP in 13 of the 30 esophageal infiltrative cancer tissues (with a mutation rate of 43.3%) and in only 1 of the 30 juxtacancerous normal tissues from the preinvasive cancer group, obvious mutation was detected by SSCP in 5 of the 14 preinvasive esophageal cancer tissues (with the mutation rate of 35.7%). In addition, obvious mutation was also detected in one specimen of hyperplasia of squamous epithelium. Within 7 esophageal cancer specimens, 58 bp (177nt to 234nt) deleted mutation of POLB was found. There were 8 point mutation forms: (1) A --> G at 375nt ( Ile --> Val) (2) T --> C at 454nt ( Phe --> Ser) (3) G --> T at 462nt (Glu --> terminal code) (4) G --> A at 466nt ( Gly --> Glu) (5) A --> T at 613nt (Lys --> Ile) (6) G --> C at 648nt (Gly --> Arg) (7) A --> G at 660nt (Arg --> Gly), and (8) A --> G at 670nt (Glu --> Gly). CONCLUSION:
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Authors | Ziming Dong, Guoqiang Zhao, Qin Zhao, Hongyan Yang, Lexun Xue, Xiaohui Tan, Naigang Zheng |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 82
Issue 13
Pg. 899-902
(Jul 2002)
ISSN: 0376-2491 [Print] China |
PMID | 12126515
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Base Sequence
- DNA Polymerase beta
(genetics)
- Esophageal Neoplasms
(genetics)
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
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