Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E.

Abstract

A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek's disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek's disease. Chickens were immunized at 1 day old with 10(3) p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek's disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.

• Authors: Daniel Schumacher, B Karsten Tischer, Jens-Peter Teifke, Kerstin Wink, Nikolaus Osterrieder
• Journal: The Journal of general virology (J Gen Virol) Vol. 83 Issue Pt 8 Pg. 1987-1992 (Aug 2002) ISSN: 0022-1317 [Print] England
• PMID: 12124462 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Viral Envelope Proteins
• Viral Vaccines
• glycoprotein E, Marek's disease virus

Topics

• Animals
• Chickens
• Mardivirus (growth & development, immunology, pathogenicity)
• Marek Disease (prevention & control, virology)
• Muscles (cytology)
• Poultry Diseases (prevention & control, virology)
• Quail
• Tumor Cells, Cultured
• Vaccination
• Viral Envelope Proteins (biosynthesis)
• Viral Vaccines
• Virology (methods)
• Virulence
• Virus Cultivation
• Virus Replication