Diagnostic DNA-flow- vs. -image-cytometry in effusion cytology.

Abstract

AIMS:

To determine the sensitivity and specificity of flow- and image-cytometry for the detection of DNA-aneuploidy as a marker for malignant cells in effusions.

METHODS:

200 effusions (80 tumor cell-positive, 74 negative and 46 cytologically equivocal) were stained with DAPI-SR for DNA-flow- and with Feulgen-Pararosaniline for -image-cytometry. They were measured using a PAS-flow-cytometer and an AutoCyte-QUIC-DNA-workstation according to the ESACP consensus reports for DNA-flow- and -image-cytometry, respectively [7,23,29,49].

RESULTS:

Sensitivity of DNA-aneuploidy for the identification of malignant cells was 32.1% for DNA-flow- and 75.0% for -image-cytometry, specificity of -euploidy in benign cells was 100.0% for both methods. Positive predictive value of DNA-aneuploidy for the identification of malignant cells was 100.0% for both techniques, negative predictive value of DNA-euploidy was 48.6% for DNA-flow- and 72.0% for -image-cytometry.

CONCLUSIONS:

Searching for DNA-aneuploidy as a diagnostic marker for neoplastic cells in serous effusions image-cytometry revealed superior sensitivity as compared with monoparametric flow cytometry.

• Authors: Helma Motherby, Natalia Pomjanski, Mary Kube, Alexandra Boros, Thomas Heiden, Bernhard Tribukait, Alfred Böcking
• Journal: Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology (Anal Cell Pathol) Vol. 24 Issue 1 Pg. 5-15 ( 2002) ISSN: 0921-8912 [Print] Netherlands
• PMID: 12122279 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• DNA

Topics

• Algorithms
• Aneuploidy
• Ascitic Fluid (pathology, physiopathology)
• Biomarkers, Tumor (analysis)
• Carcinoma (pathology, physiopathology)
• DNA (analysis)
• Data Interpretation, Statistical
• Epithelium (pathology, physiopathology)
• Flow Cytometry (instrumentation, methods)
• Humans
• Image Cytometry (instrumentation, methods)
• Mesothelioma (pathology, physiopathology)
• Pleural Effusion, Malignant (pathology, physiopathology)
• Reproducibility of Results
• Serous Membrane (pathology, physiopathology)