Effects of spiramine T on antioxidant enzymatic activities and nitric oxide production in cerebral ischemia-reperfusion gerbils.

Abstract

Spiramine T, an atisine-type diterpene alkaloid isolated from the Chinese herbal medicine Spiraea japonica var. acuta (Rosaceae), was shown to have neuroprotective effects on cerebral ischemia-reperfusion injury. In this study, the effects of spiramine T on antioxidant enzymes and nitric oxide production were evaluated in gerbils subjected to global forebrain ischemia (10 min) and reperfusion (5 days). Spiramine T (1.0 and 2.0 mg kg(-1) i.p.) markedly reduced the content of lipid peroxide (LPO), increased the glutathione peroxidase (GSH-PX) activity, and inhibited the increase of nitric oxidase (NOS) activity and nitric oxide production in the cortex during ischemia-reperfusion in gerbils. These results suggested that the neuroprotective effects of spiramine T were related to modulation of endogenous antioxidant enzymatic activities and reduction of the formation of nitric oxide.

• Authors: Ling Li, Yue-Mao Shen, Xiao-Sheng Yang, Wan-Ling Wu, Bin-Gui Wang, Zhi-He Chen, Xiao-Jiang Hao
• Journal: Brain research (Brain Res) Vol. 944 Issue 1-2 Pg. 205-9 (Jul 19 2002) ISSN: 0006-8993 [Print] Netherlands
• PMID: 12106682 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Free Radical Scavengers
• Heterocyclic Compounds, 4 or More Rings
• Lipid Peroxides
• spiramine T
• Nitric Oxide
• Malondialdehyde
• Catalase
• Glutathione Peroxidase
• Nitric Oxide Synthase
• Superoxide Dismutase
• Glutathione

Topics

• Animals
• Antioxidants (pharmacology)
• Brain Ischemia (drug therapy, enzymology, physiopathology)
• Catalase (drug effects, metabolism)
• Cerebral Cortex (drug effects, enzymology, physiopathology)
• Drugs, Chinese Herbal (pharmacology)
• Female
• Free Radical Scavengers (pharmacology)
• Gerbillinae
• Glutathione (metabolism)
• Glutathione Peroxidase (drug effects, metabolism)
• Heterocyclic Compounds, 4 or More Rings (pharmacology)
• Lipid Peroxides (metabolism)
• Male
• Malondialdehyde (metabolism)
• Neurons (drug effects, enzymology)
• Nitric Oxide (biosynthesis)
• Nitric Oxide Synthase (drug effects, metabolism)
• Oxidative Stress (drug effects, physiology)
• Reperfusion Injury (drug therapy, enzymology, physiopathology)
• Superoxide Dismutase (drug effects, metabolism)