Cloning and characterization of a novel haemolysin in Vibrio cholerae O1 that does not directly contribute to the virulence of the organism.

Abstract

A previously undescribed haemolysin, distinct from the major Vibrio cholerae O1 El Tor haemolysin, HlyA, was cloned from the O1 classical biotype strain Z17561. This novel haemolysin showed 71.5% overall similarity to the delta-thermostable direct haemolysin of Vibrio parahaemolyticus, and so it has been termed V. cholerae delta-thermostable haemolysin (Vc-deltaTH, encoded by the dth gene). An ORF found immediately downstream, which appears to be transcriptionally and translationally linked to dth, displayed strong homology to the family of acyl-CoA synthetases. When expressed from an inducible promoter in Escherichia coli, Vc-deltaTH was shown to be a 22.8 kDa protein active on sheep red blood cells. Co-expression of acs with dth had no effect on the haemolytic activity or cytoplasmic localization of Vc-deltaTH. A V. cholerae Z17561 dth::Km(R) mutant showed unaltered behaviour in the infant mouse cholera model.

• Authors: Angelo Fallarino, Stephen R Attridge, Paul A Manning, Tony Focareta
• Journal: Microbiology (Reading, England) (Microbiology (Reading)) Vol. 148 Issue Pt 7 Pg. 2181-2189 (Jul 2002) ISSN: 1350-0872 [Print] England
• PMID: 12101305 (Publication Type: Journal Article)

Chemical References

• Hemolysin Proteins

Topics

• Amino Acid Sequence
• Animals
• Animals, Newborn
• Cholera (microbiology, physiopathology)
• Cloning, Molecular
• Disease Models, Animal
• Hemagglutination Tests
• Hemolysin Proteins (genetics, metabolism)
• Humans
• Mice
• Molecular Sequence Data
• Mutation
• Sequence Analysis, DNA
• Subcellular Fractions (metabolism)
• Vibrio cholerae (genetics, metabolism, pathogenicity)
• Virulence