An association study between the Clara cell secretory protein CC16 A38G polymorphism and asthma phenotypes.

Abstract

BACKGROUND:

Previously, an association has been reported between an increased risk of asthma and a polymorphism in the Clara cell secretory protein (CC16) gene [namely, an adenine to guanine substitution in the CC16 gene at position 38 (A38G) downstream from the transcription initiation site within the noncoding region of exon 1]. Homozygous individuals for the polymorphic sequence (AA genotype) were reported to have a significant (6.9 fold) increased risk of developing asthma. This finding has not been confirmed independently.

OBJECTIVE:

To validate the association of CC16 A38G polymorphism to asthma in a separate well-characterized population through a case-control study.

METHODS:

We conducted an association study using a sample of 217 unrelated Northern European Caucasians. Individuals were clinically characterized by a validated respiratory questionnaire, spirometry and bronchial reactivity measurement, and genotyped for the A38G polymorphism using PCR and restriction digestion. Association analysis was performed using the nonparametric Chi-squared tests.

RESULTS:

In the unselected population, 43.3% participants were homozygous for the CC16*G allele and 45.4% were heterozygous (AG). We observed no significant difference in the distribution of positive bronchial reactivity to methacholine (at FEV1 PC20 of </= 8 mg/mL) across the three genotypes. Homozygous individuals for the CC16*A allele did not demonstrate an increased risk of asthma when compared to heterozygous or GG homozygotes. In addition, no significant difference was observed in the distribution of the CC16*A or *G alleles in the asthmatics vs. non-asthmatics.

CONCLUSION:

CC16 polymorphism A38G does not influence the predisposition to asthma in this sample.

• Authors: A H Mansur, A A Fryer, M Hepple, R C Strange, M A Spiteri
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 32 Issue 7 Pg. 994-9 (Jul 2002) ISSN: 0954-7894 [Print] England
• PMID: 12100044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteins
• SCGB1A1 protein, human
• Uteroglobin

Topics

• Adolescent
• Adult
• Asthma (genetics)
• Bronchial Hyperreactivity (genetics)
• Chromosome Mapping
• Female
• Genotype
• Humans
• Male
• Middle Aged
• Phenotype
• Polymorphism, Genetic
• Proteins (genetics)
• Uteroglobin