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Dissimilar invasive and metastatic behavior of vincristine and doxorubicin-resistant cell lines derived from a murine T cell lymphoid leukemia.

Abstract
Multidrug resistance (MDR) lines from a murine T-cell lymphoid leukemia were selected in increasing vincristine (VCR) or doxorubicin (DOX) concentrations. Surface markers were determined by flow cytometry in both resistant (LBR-V 160 and LBR-D 160) and sensitive (LBR-) cell lines. Results obtained revealed similar expression of CD25, CD24, CD8, CD4, C18 and CD44, while differences in binding to hyaluronic acid (HA) were found. LBR- and LBR-D 160 bound to HA only after phorbol ester (PMA) activation, while LBR-V160 failed to bind HA even after PMA treatment. Histopathological analysis disclosed that LBR-V160 was less invasive than LBR- and LBR-D160 cell lines. In vitro growth of cell lines analyzed by sulforhodamine-B uptake showed that doubling time for the three lines was 10.24 h (LBR-), 16.75 h (LBR-V160) and 16.29 h (LBR-D160). Mortality rate was determined after i.p. injection of 10(4) cells. Mice inoculated with LBR- died at 23 2.11) days, while those inoculated with LBR-V160 or LBR-D160 died at 41 (+/- 9.53) or 41 (+/- 4.96) days, respectively. Our results demonstrated that leukemic murine T cells cultured in the long-term presence of VCR or DOX not only presented changes in the resistance phenotype but also variations in their growth and metastatic pattern.
AuthorsEloisi C Lopes, Glenda Ernst, Paula Aulicino, Silvia Vanzulli, Mariana García, Elida Alvarez, Silvia E Hajos
JournalClinical & experimental metastasis (Clin Exp Metastasis) Vol. 19 Issue 4 Pg. 283-90 ( 2002) ISSN: 0262-0898 [Print] Netherlands
PMID12090468 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibiotics, Antineoplastic
  • Antineoplastic Agents, Phytogenic
  • Hyaluronan Receptors
  • Neoplasm Proteins
  • Vincristine
  • Doxorubicin
  • Hyaluronic Acid
  • Tetradecanoylphorbol Acetate
Topics
  • Animals
  • Antibiotics, Antineoplastic (pharmacology)
  • Antineoplastic Agents, Phytogenic (pharmacology)
  • Cell Division
  • Doxorubicin (pharmacology)
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Hyaluronan Receptors (metabolism)
  • Hyaluronic Acid (metabolism)
  • Lymphoma, T-Cell (pathology)
  • Mice
  • Neoplasm Invasiveness
  • Neoplasm Metastasis
  • Neoplasm Proteins (metabolism)
  • Neoplasm Transplantation
  • Tetradecanoylphorbol Acetate (pharmacology)
  • Tumor Cells, Cultured (drug effects, physiology)
  • Vincristine (pharmacology)

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