Abstract |
Multidrug resistance (MDR) lines from a murine T-cell lymphoid leukemia were selected in increasing vincristine (VCR) or doxorubicin (DOX) concentrations. Surface markers were determined by flow cytometry in both resistant (LBR-V 160 and LBR-D 160) and sensitive (LBR-) cell lines. Results obtained revealed similar expression of CD25, CD24, CD8, CD4, C18 and CD44, while differences in binding to hyaluronic acid (HA) were found. LBR- and LBR-D 160 bound to HA only after phorbol ester (PMA) activation, while LBR-V160 failed to bind HA even after PMA treatment. Histopathological analysis disclosed that LBR-V160 was less invasive than LBR- and LBR-D160 cell lines. In vitro growth of cell lines analyzed by sulforhodamine-B uptake showed that doubling time for the three lines was 10.24 h (LBR-), 16.75 h (LBR-V160) and 16.29 h (LBR-D160). Mortality rate was determined after i.p. injection of 10(4) cells. Mice inoculated with LBR- died at 23 2.11) days, while those inoculated with LBR-V160 or LBR-D160 died at 41 (+/- 9.53) or 41 (+/- 4.96) days, respectively. Our results demonstrated that leukemic murine T cells cultured in the long-term presence of VCR or DOX not only presented changes in the resistance phenotype but also variations in their growth and metastatic pattern.
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Authors | Eloisi C Lopes, Glenda Ernst, Paula Aulicino, Silvia Vanzulli, Mariana García, Elida Alvarez, Silvia E Hajos |
Journal | Clinical & experimental metastasis
(Clin Exp Metastasis)
Vol. 19
Issue 4
Pg. 283-90
( 2002)
ISSN: 0262-0898 [Print] Netherlands |
PMID | 12090468
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- Antineoplastic Agents, Phytogenic
- Hyaluronan Receptors
- Neoplasm Proteins
- Vincristine
- Doxorubicin
- Hyaluronic Acid
- Tetradecanoylphorbol Acetate
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Topics |
- Animals
- Antibiotics, Antineoplastic
(pharmacology)
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Cell Division
- Doxorubicin
(pharmacology)
- Drug Resistance, Multiple
- Drug Resistance, Neoplasm
- Hyaluronan Receptors
(metabolism)
- Hyaluronic Acid
(metabolism)
- Lymphoma, T-Cell
(pathology)
- Mice
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Neoplasm Proteins
(metabolism)
- Neoplasm Transplantation
- Tetradecanoylphorbol Acetate
(pharmacology)
- Tumor Cells, Cultured
(drug effects, physiology)
- Vincristine
(pharmacology)
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