HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Monoclonal antibody (5F3) defining renal cell carcinoma-associated antigen disialosyl globopentaosylceramide (V3NeuAcIV6NeuAcGb5), and distribution pattern of the antigen in tumor and normal tissues.

Abstract
Renal cell carcinoma (RCC) has been characterized by high expression of three types of disialogangliosides: two based on lacto-series type 1 structure (disialosyl Lc(4), GalNAc disialosyl Lc(4)), the other based on globo-series structure (disialosyl globopentaosylceramide; disialosyl Gb5). The present study established a mAb, 5F3, directed to disialosyl Gb5. 5F3 was established after immunization with RCC cell line ACHN. The major disialoganglioside antigen isolated from ACHN cells, showing specific reactivity with 5F3, was characterized unequivocally as disialosyl Gb5 (V(3)NeuAcIV(6)NeuAcGb5) by identification of the core structure as globopentaosylceramide (Gb5) after enzymatic and acid hydrolysis, and by 2-dimensional (1)H-NMR spectroscopy. 5F3 does not react with monosialosyl Gb5 (V(3)NeuAcGb5), Gb5, or any lacto-series structures. 5F3 strongly stained 19 of 41 cases of primary RCC tissue. It reacted with proximal tubules (but not distal tubules) of kidney, microglial cells of cerebrum and cerebellum, goblet cells of stomach and intestine, smooth muscle of various organs. It did not react with parenchymatous cells of various organs, except for kidney epithelia and prostate stroma. Immunostaining of RCC tissue by mAb 5F3, in combination with staining by other antibodies directed to globo-series and lacto-series structures, has prognostic significance in defining metastatic potential of RCC.
AuthorsA Ito, S Saito, T Masuko, M Oh-Eda, T Matsuura, M Satoh, F M Nejad, T Enomoto, S Orikasa, S I Hakomori
JournalGlycoconjugate journal (Glycoconj J) Vol. 18 Issue 6 Pg. 475-85 (Jun 2001) ISSN: 0282-0080 [Print] United States
PMID12084983 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Gangliosides
  • Glycosphingolipids
  • sialogangliosides
Topics
  • Antibodies, Monoclonal (immunology)
  • Antibody Specificity
  • Antigens, Neoplasm (immunology)
  • Carcinoma, Renal Cell (chemistry, diagnosis, immunology, pathology)
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Gangliosides (analysis, immunology)
  • Glycosphingolipids (immunology)
  • Humans
  • Immunohistochemistry
  • Magnetic Resonance Spectroscopy
  • Neoplasm Metastasis
  • Organ Specificity
  • Prognosis
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: