Quantitation of latex allergens.

Abstract

Minimizing allergen concentration in latex goods to prevent sensitization to natural rubber latex (NRL) and thereby the development of clinical allergy is acknowledged as of mutual interest for rubber manufacturers and regulatory health authorities. However, measuring total protein, the principal currently available method, cannot be deemed a satisfactory regulatory measure to control allergen content. Specific methods based on human IgE-containing reagents, such as radioallergosorbent test (RAST) inhibition, have been available in certain laboratories for demonstrating NRL allergens in rubber products but the methods lack standardization. Currently, one commercial test has become available for measuring individual NRL allergens by capture ELISA-based assays using monoclonal antibodies and purified or recombinant allergens. Such methods are specific, they can be properly standardized, and they are of sufficient sensitivity and reproducibility. Results from medical gloves collected in two national market surveys in Finland in 1995 and 1999, respectively, show that Hev b 6.02 and Hev b 5, the two major allergens for NRL-allergic adults, are the most abundant allergens regularly detectable in high- and moderate-allergen gloves. In addition, Hev b 3 and Hev b 1, the two major allergens for children with spina bifida, are also commonly found. In general, when the sum of the four allergens exceeded 1 microg/g, most NRL-allergic patients showed positive skin prick test reactions against them. Using these new methods assessment of threshold levels that could in due course become guidelines for the rubber industry and regulatory health authorities is becoming possible. Eventually, this progress is expected to lead to a declining incidence of latex allergy.

• Authors: Timo Palosuo, Harri Alenius, Kristiina Turjanmaa
• Journal: Methods (San Diego, Calif.) (Methods) Vol. 27 Issue 1 Pg. 52-8 (May 2002) ISSN: 1046-2023 [Print] United States
• PMID: 12079417 (Publication Type: Journal Article, Review)
• Copyright: Copyright 2002 Elsevier Science (USA).

Chemical References

• Allergens
• Antibodies, Monoclonal
• Latex

Topics

• Allergens (analysis, immunology)
• Antibodies, Monoclonal
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Latex (analysis, immunology)
• Latex Hypersensitivity (immunology)