Corneal endothelial cell loss after nonmechanical penetrating keratoplasty depends on diagnosis: a regression analysis.

Abstract

PURPOSE:

To assess the impact of diagnosis on corneal endothelial cell loss after nonmechanical penetrating keratoplasty (PK) with regression models.

METHODS:

Five hundred eighty-nine eyes [273 with keratoconus (group I; PK only), 187 with Fuchs' endothelial dystrophy (group II; 77 PK only and 110 triple procedures) and 129 with bullous keratopathy (group III; 115 pseudophakic and 14 aphakic eyes)] were included in this prospective study. The time course of the endothelial cell density after PK was assessed by specular microscopy. Endothelial cell density was analyzed in cross-sectional fashion at 6 and 24 months follow-up and in longitudinal manner (follow-up 2.4+/-1.8 years) with linear and exponential regression models (minimizing the residuum between observed and predicted endothelial cell count). Donor age, post-mortem time, storage time and ratio of organ-cultured to short-term-preserved donor corneas did not differ significantly between groups of patients.

RESULTS:

In a cross section, endothelial cell density in group I / group II / group III decreased from 1,959+/-499 / 1,524+/-528 / 1,526+/-670 cells/mm(2)to 1,617+/-553 / 1,222+/-520 / 1,063+/-384 cells/mm(2) (P=0.06 / 0.04 / 0.005). Endothelial cell count was significantly higher in group I than in group II (P=0.048) and significantly higher in group II than in group III (P=0.01) at 24 months. In the linear regression model, cell count decreased in group I / group II / group III by 136+/-465 / 241+/-374 / 421+/-484 cells annually. In the exponential regression model, cell count decreased in group I / group II / group III by 2.9+/-28.0% / 11.2+/-21.8% / 19.3+/-29.4% annually. Cell loss was highly significantly lower in group I than in group II ( P<0.0001) and highly significantly lower in group II than in group III (P<0.0001).

CONCLUSION:

Endothelial cell loss seems to be least pronounced after PK in keratoconus (2.9%), followed by Fuchs' dystrophy (11.2%) and bullous keratopathy (19.3%). These differences between diagnoses indicate migration of endothelial cells along a density gradient after PK.

• Authors: Achim Langenbucher, Berthold Seitz, Nhung X Nguyen, Gottfried O H Naumann
• Journal: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (Graefes Arch Clin Exp Ophthalmol) Vol. 240 Issue 5 Pg. 387-92 (May 2002) ISSN: 0721-832X [Print] Germany
• PMID: 12073062 (Publication Type: Comparative Study, Journal Article)

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Cell Count
• Cell Survival
• Child
• Corneal Diseases (diagnosis, surgery)
• Endothelium, Corneal (pathology)
• Female
• Follow-Up Studies
• Humans
• Keratoplasty, Penetrating
• Male
• Middle Aged
• Prospective Studies
• Regression Analysis
• Time Factors
• Tissue Donors