Inhibition of cell cycle progression in human leukemia HL-60 cells by esculetin.

Abstract

Esculetin, a coumarin compound, was found to inhibit cell growth and cell cycle progression by inducing arrest of the G1 phase in HL-60 cells. To obtain information regarding cell cycle arrest induced by esculetin, we examined its effect on the regulating factors of the G1 phase in the leukemia HL-60 cells treated with esculetin by Western blotting. Our observations were: (1) a distinct increase in the level of hypophosphorylated retinoblastoma protein (pRb), and a reduction in the level of CDK4 after treatment with 100 microM of esculetin for 24 h; (2) a marked up-regulation of p27, and a down-regulation of cyclin D1 after treatment with 100 microM esculetin for 24 h. These results suggest that esculetin can inhibit the growth of human leukemia HL-60 cells by G1 phase cell cycle arrest as a result of inhibited pRb phosphorylation.

• Authors: Chau Jong Wang, Yu Jin Hsieh, Chia Yih Chu, Yu Ling Lin, Tsui Hwa Tseng
• Journal: Cancer letters (Cancer Lett) Vol. 183 Issue 2 Pg. 163-8 (Sep 26 2002) ISSN: 0304-3835 [Print] Ireland
• PMID: 12065091 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Antioxidants
• Microfilament Proteins
• Muscle Proteins
• Retinoblastoma Protein
• Tagln protein, mouse
• Umbelliferones
• Cyclin D1
• DNA
• esculetin

Topics

• Antineoplastic Agents (pharmacology)
• Antioxidants (pharmacology)
• Blotting, Western
• Cell Cycle (drug effects)
• Cell Line
• Cyclin D1 (metabolism)
• DNA (metabolism)
• Down-Regulation
• HL-60 Cells
• Humans
• Microfilament Proteins (metabolism)
• Muscle Proteins
• Phosphorylation
• Retinoblastoma Protein (metabolism)
• Time Factors
• Umbelliferones (pharmacology)
• Up-Regulation